Raffinose-Carboxymethyl Cellulose Hydrogel, Silk Fibroin, and Agbtc Mof: A Nanobiocomposite With Biomedical Applications Publisher

Summary: Can a natural gel fight infections? Research shows a raffinose-based hydrogel with silver kills cancer cells and bacteria, promising for healing and therapy. #Biomedicine #Antimicrobial

Nia SM¹ ; Naderi N¹ ; Aliabadi HAM² ; Kashtiaray A¹ ; Mahdavi M³ ; Eivazzadehkeihan R⁴ ; Maleki A¹
Source: Cellulose Published:2024

Abstract

Natural polymer-based hydrogels have importance in tissue engineering, drug delivery systems, and wound dressings due to their non-toxicity, renewability, biocompatibility, and biodegradability. Also, hydrogels can be modified to increase their antibacterial activity and mechanical properties. In this study, a novel nanobiocomposite was fabricated using raffinose (Raff)-carboxymethyl cellulose (CMC) hydrogel, silk fibroin (SF), and AgBTC metal organic framework (MOF). The nanobiocomposite was characterized using Fourier transform infrared (FT-IR), field emission scanning electron microscopy (FE-SEM), energy dispersive X-ray (EDX), thermogravimetric analysis (TGA), and X-ray diffraction (XRD) analyses. After 48 h of incubation, the (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide) (MTT) assay of Raff-CMC hydrogel/SF/AgBTC nanobiocomposite on HEK293T cells (human embryonic healthy kidney cell lines) and MCF-7 cells (human breast cancer cell lines) showed that the percentage of cell viability of the two cell lines was 90.46% and 29.18%, respectively. The measured hemolytic activity of the nanobiocomposite on human red blood cells was 2.39%, indicating its safety for use in the human circulatory system, as it did not cause significant hemolysis in erythrocytes compared to the negative control. The potential of the Raff-CMC hydrogel/SF/AgBTC nanobiocomposite to inhibit bacterial growth was investigated, and results showed that E. coli and S. aureus growth was restricted by 60.38% and 57.09%, respectively. As a result, considering the biocompatibility of nanobiocomposite with healthy cells, its antibacterial and anticancer activity, as well as its hemocompatibility, it can be considered a potential candidate for biomedical applications such as wound healing, tissue engineering, and cancer therapy. © The Author(s), under exclusive licence to Springer Nature B.V. 2024.