Functionalized Graphene Oxide Nanosheets With Folic Acid and Silk Fibroin As a Novel Nanobiocomposite for Biomedical Applications Publisher Pubmed



Eivazzadehkeihan R¹ ; Alimirzaloo F¹ ; Aghamirza Moghim Aliabadi H^{2, 3} ; Bahojb Noruzi E⁴ ; Akbarzadeh AR⁵ ; Maleki A¹ ; Madanchi H^{6, 7} ; Mahdavi M⁸
Source: Scientific Reports Published:2022

Abstract

In this paper, a novel graphene oxide-folic acid/silk fibroin (GO-FA/SF) nanobiocomposite scaffold was designed and fabricated using affordable and non-toxic materials. The GO was synthesized using the hummer method, covalently functionalized with FA, and then easily conjugated with extracted SF via the freeze-drying process. For characterization of the scaffold, several techniques were employed: Fourier-transform infrared (FT-IR), field emission scanning electron microscopy (FE-SEM), energy dispersive X-ray (EDX), and thermogravimetric analysis (TGA). The cell viability method, hemolysis, and anti-biofilm assays were performed, exploring the biological capability of the nanobiocomposite. The cell viability percentages were 96.67, 96.35 and 97.23% for 24, 48, and 72 h, respectively, and its hemolytic effect was less than 10%. In addition, it was shown that this nanobiocomposite prevents the formation of Pseudomonas aeruginosa biofilm and has antibacterial activity. © 2022, The Author(s).