Distribution of Il-28B Genotypes in Patients With Hepatitis C and Healthy Individuals in Jahrom City



Mousavi Nasab SD¹ ; Baharlou R³ ; Piroozmand A^{1, 2} ; Toghyani H⁴ ; Shadmand E³ ; Fazel H⁴ ; Sadeghi K⁴ ; Ali Hashemi SM³ ; Shokouh MR³ ; Gheshlaghi A³ ; Ahmadi NA⁵ ; Vasmehjani AA³
Source: Gastroenterology and Hepatology from Bed to Bench Published:2015

Abstract

Aim: The purpose of this study was to compare the distribution of interleukin (IL)-28B genotypes between Iranian healthy individuals and patients with chronic hepatitis C based on the genotype. Background: Polymorphisms in the region of IL-28B gene have been identified as the strongest genetic pretreatment predictor of sustained virological response (SVR) in hepatitis C infection. Patients and methods: In this study, 147 patients with chronic hepatitis C and 80 healthy individuals were included. The IL-28B rs12979860 and rs8099917 polymorphisms were genotyped by PCR-RFLP method and the frequency of IL-28B polymorphisms with respect to HCV genotypes was also determined. Results: The frequencies of rs12979860 TT, CC and CT genotypes in the chronic hepatitis C patients and healthy individuals were as follows: 10.8% vs. 11.3%, 38.7% vs. 46.2% and 50.3% vs. 42.5%. Also, the frequencies of rs8099917 TT, GG and GT genotypes in the chronic hepatitis C patients was 61.9%, 6.1% and 32% and in controls was 47.5%, 11.2% and 41.3%. The differences in the distribution of rs12979860 genotypes and alleles between HCV genotype 1 and HCV genotype 3a infected patients were statistically significant. Conclusion: The rs12979860 C allele is the favorable allele for the spontaneous clearance of HCV. It seems that the impact of IL-28B polymorphism on the spontaneous clearance of HCV genotype 3 is more prominent than HCV genotype 1, which results in the observation of higher rs12979860 C allele frequency in chronic hepatitis C patients with HCV genotype 3 than HCV genotype 1. i¿½ 2015 RIGLD, Research Institute for Gastroenterology and Liver Diseases.