Association of Il-10 and Tgf-Beta Cytokine Gene Polymorphisms With Autoimmune Hepatitis Publisher Pubmed



Yousefi A¹ ; Zare Bidoki A² ; Shafioyoun A² ; Sadr M² ; Varzaneh FN² ; Shabani M^{3, 4} ; Motamed F⁵ ; Farahmand F⁵ ; Khodadad A⁵ ; Fallahi G⁵ ; Najafi M⁵ ; Rezaei N^{3, 6, 7}
Source: Clinics and Research in Hepatology and Gastroenterology Published:2019

Abstract

Background and aims: Autoimmune hepatitis is a chronic immune-mediated liver injury caused by dysregulated immune response to liver antigens. Genetic susceptibility is affected by multiple single nucleotide polymorphisms in immune-related genes. There are few reports on the association of TGF-β and IL-10 genetic variants with autoimmune hepatitis. Methods: Allele frequency and genotype status of IL-10 −1082, −819, −592 and TGF-β +869 and +915 polymorphisms were investigated in 57 unrelated patients with autoimmune hepatitis and 140 healthy controls by polymerase chain reaction with sequence-specific primers. Results: IL-10 −592 and −819 allele frequencies and genotypes were not associated with autoimmune hepatitis in our population, while IL-10 −1082 genotypes were. IL-10 −1082/−819/−592 “high-producing” haplotype GCC was significantly less frequent in patients. TGF-β +869 “high-producing” allele C and genotype CC were significantly more in autoimmune hepatitis, compared to controls; whereas, TGF-β +915 “low-producing” allele C and genotype CC were significantly more in autoimmune hepatitis compared to control. TGF-β +869/+915 haplotype TG was significantly less frequent in patients while CC haplotype was significantly more frequently observed in patients. Conclusion: We identified a significant association between IL-10 −1082/−819 and TGF-β +869/+915 genotypes and haplotypes with autoimmune hepatitis in Iranians. © 2018 Elsevier Masson SAS