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Hydrogel Nanoparticle Encapsulated Plasmid As a Suitable Gene Delivery System Publisher



Rafiee A1 ; Riazirad F2 ; Alimohammadian MH2 ; Gazori T3 ; Fatemi SMR4 ; Havaskary M5
Authors

Source: Cytology and Genetics Published:2015


Abstract

To facilitate the delivery of genetic material, the use of appropriate carriers such as polymers is necessary. Nanoparticles comprising of chitosan-alginate polymers were formed through pregel preparation method. Chi/Alg nanoparticles had a mean Z-Average diameter of 161.8 nm and mean zeta 29.3 mV, respectively. The ability of plasmid-complex in preventing DNA migration showed Chi/Alg nanoparticles have great capacity to maintain plasmid. The efficiency of nanoparticles for transfection of pEGFP-N1 plasmid in the cultured HEK 293 cells was measured by flow cytometry. Cell viability assays indicated that nanoparticles had no toxic effect on HEK 293 cells after 4 or 24 h. Our suitable candidate for gene delivery would be alg/chi nanoparticles. © 2015, Allerton Press, Inc.
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