Chitosan Nanoparticles As a Potential Nonviral Gene Delivery for Hpv-16 E7 Into Mammalian Cells

Chitosan Nanoparticles As a Potential Nonviral Gene Delivery for Hpv-16 E7 Into Mammalian Cells Publisher Pubmed

Tahamtan A^{1, 2} ; Tabarraei A¹ ; Moradi A¹ ; Dinarvand M³ ; Kelishadi M¹ ; Ghaemi A¹ ; Atyabi F⁴

Source: Artificial Cells# Nanomedicine and Biotechnology Published:2015

Abstract

Chitosan nanoparticles (CS NPs) were prepared as a carrier for Human papillomavirus type 16 HPV-16) E7 gene and their gene transfection ability were evaluated in vitro . The plasmid expressing green fl uorescent protein (pEGFP) was used as a reporter gene. Gel electrophoresis demonstrated full binding of CS NPs with the pDNA. The transfection of CS-pEGFP NPs was effi cient in CHO cells and the expression of green fl uorescent proteins was well observed. The expression of E7 proteins was confi rmed under SDS-PAGE and western blot analysis. As a conclusion CS NPs may serve as an eff ective nonviral carrier for delivery of nucleotides into eukaryotic cells. Copyright © 2014 Informa Healthcare USA, Inc.

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Fatemeh Atyabi (3)

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Style	Citing Format
MLA	Tahamtan A, et al.. "Chitosan Nanoparticles As a Potential Nonviral Gene Delivery for Hpv-16 E7 Into Mammalian Cells." Artificial Cells# Nanomedicine and Biotechnology, vol. 43, no. 6, 2015, pp. 366-372.
APA	Tahamtan A, Tabarraei A, Moradi A, Dinarvand M, Kelishadi M, Ghaemi A, Atyabi F (2015). Chitosan Nanoparticles As a Potential Nonviral Gene Delivery for Hpv-16 E7 Into Mammalian Cells. Artificial Cells# Nanomedicine and Biotechnology, 43(6), 366-372.
Chicago	Tahamtan A, Tabarraei A, Moradi A, Dinarvand M, Kelishadi M, Ghaemi A, Atyabi F. "Chitosan Nanoparticles As a Potential Nonviral Gene Delivery for Hpv-16 E7 Into Mammalian Cells." Artificial Cells# Nanomedicine and Biotechnology 43, no. 6 (2015): 366-372.
Harvard	Tahamtan A et al. (2015) 'Chitosan Nanoparticles As a Potential Nonviral Gene Delivery for Hpv-16 E7 Into Mammalian Cells', Artificial Cells# Nanomedicine and Biotechnology, 43(6), pp. 366-372.
Vancouver	Tahamtan A, Tabarraei A, Moradi A, Dinarvand M, Kelishadi M, Ghaemi A, et al.. Chitosan Nanoparticles As a Potential Nonviral Gene Delivery for Hpv-16 E7 Into Mammalian Cells. Artificial Cells# Nanomedicine and Biotechnology. 2015;43(6):366-372.
BibTex	@article{ author = {Tahamtan A and Tabarraei A and Moradi A and Dinarvand M and Kelishadi M and Ghaemi A and Atyabi F}, title = {Chitosan Nanoparticles As a Potential Nonviral Gene Delivery for Hpv-16 E7 Into Mammalian Cells}, journal = {Artificial Cells# Nanomedicine and Biotechnology}, volume = {43}, number = {6}, pages = {366-372}, year = {2015} }
RIS	TY - JOUR AU - Tahamtan A AU - Tabarraei A AU - Moradi A AU - Dinarvand M AU - Kelishadi M AU - Ghaemi A AU - Atyabi F TI - Chitosan Nanoparticles As a Potential Nonviral Gene Delivery for Hpv-16 E7 Into Mammalian Cells JO - Artificial Cells# Nanomedicine and Biotechnology VL - 43 IS - 6 SP - 366 EP - 372 PY - 2015 ER -

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