Nano Polyelectrolyte Complexes of Carboxymethyl Dextran and Chitosan to Improve Chitosan-Mediated Delivery of Mir-145 Publisher Pubmed



Tekie FSM¹ ; Kiani M² ; Zakerian A³ ; Pilevarian F¹ ; Assali A² ; Soleimani M^{4, 5} ; Dinarvand R^{1, 2} ; Arefian E^{4, 6} ; Atashi A⁷ ; Amini M⁸ ; Atyabi F^{1, 2}
Source: Carbohydrate Polymers Published:2017

Abstract

Chitosan (Ch) nanoparticles have emerged as a promising vector for gene delivery, nonetheless slow dissociation rate of the nanoparticles in cytoplasm is a drawback of using Ch. Herein, the Ch-mediated gene delivery was improved using PECs of Ch and carboxymethyl dextran (CMD) to transfer the micro RNA-145 (miR-145). The optimized nano PEC preparation method and effects of Ch molecular weight (Mw) and a CMD to Ch molar ratio (CMD:Ch) on physical characteristics and in vitro efficacy of the nano PECs was determined. The size of the nano PECs depended on the preparation method, Ch Mw, and CMD:Ch ratio. Also, the Ch Mw and CMD:Ch affected stability of the miR-145 PECs in presence of heparin. In vitro tests indicated the different gene transfection efficiency of the nano PECs with various compositions. As a novel vector, these nano PECs have excellent promise for gene delivery with an adequate balance between a complex stability and dissociation rate. © 2016 Elsevier Ltd