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A Doxycycline-Loaded Microfiber of Poly-Metformin/Pcl for Eradicating Melanoma Stem Cells Publisher Pubmed

Summary: Can a patch stop cancer regrowth? Research shows a doxycycline-loaded skin patch kills melanoma cancer stem cells, promising post-surgery treatment. #CancerTherapy #Melanoma

Zarei B1 ; Akrami M2, 3 ; Rezaei N4 ; Mahdavi M5 ; Kamankesh M6 ; Haririan I2, 3, 7 ; Asadi M8 ; Navaeinigjeh M9
Authors

Source: International Journal of Pharmaceutics Published:2024


Abstract

Nowadays, electrospun fibrous mats are used as drug delivery systems for loading of potential drugs in order to kill cancer cells. In the study, a skin patch for treating melanoma cancer after surgery was made using polycaprolactone and polymetformin microfibers that were loaded with doxycycline (PolyMet/PCL@DOX), an anti-cancer stem cell agent. The morphology, structure, mechanical characteristics, swelling, and porosity of the electrospun microfibers were examined. Drug release and anticancer effectiveness of PolyMet/PCL@DOX was evaluated against A375 melanoma cancer stem cells using the MTS, Flow cytometry, colony formation and CD44 expression assays. Scanning electron microscopy (SEM) verified the micro fibrous structure with a diameter of about 2.31 µm. The porosity and swelling percentages for microfibers was 73.5 % and 2.9 %, respectively. The tensile strength at the breaking point was equal to 3.84 MPa. The IC50 of PolyMet/PCL@DOX was 7.4 μg/mL. The survival rate of A375 cells after 72 h of PolyMet/PCL@DOX treatment was 43.9 %. The colony formation capacity of A375 cells decreased after PolyMet/PCL@DOX treatment. The level of CD44 expression in the PolyMet/PCL@DOX group decreased compared to the control group. Generally, PolyMet/PCL@DOX microfibers can be a promising candidate as a patch after surgery to eradicate cancer stem cells, effectively. © 2024
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A Doxycycline-Loaded Microfiber of Poly-Metformin/Pcl for Eradicating Melanoma Stem Cells
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