High Porous Electrospun Poly(Ε-Caprolactone)/Gelatin/Mgo Scaffolds Preseeded With Endometrial Stem Cells Promote Tissue Regeneration in Full-Thickness Skin Wounds: An in Vivo Study Publisher Pubmed



Ababzadeh S^{1, 2} ; Farzin A^{3, 4} ; Goodarzi A⁵ ; Karimi R⁴ ; Sagharjoghi Farahani M² ; Eslami Farsani M⁶ ; Gharibzad K⁷ ; Zahiri M⁸ ; Ai J^{2, 4}
Source: Journal of Biomedical Materials Research - Part B Applied Biomaterials Published:2020

Abstract

In the current study, electrospun poly(ε-caprolactone)-gelatin (PCL-Gel) fibrous scaffolds containing magnesium oxide (MgO) particles and preseeded with human endometrial stem cells (hEnSCs) were developed to use as wound care material in skin tissue engineering applications. Electrospun fibers were fabricated using PCL-Gel (1:1 [wt/wt]) with different concentrations of MgO particles (1, 2, and 4 wt%). The fibrous scaffolds were evaluated regarding their microstructure, mechanical properties, surface wettability, and in vitro and in vivo performances. The full-thickness excisional wound model was used to evaluate the in vivo wound healing ability of the fabricated scaffolds. Our findings confirmed that the wounds covered with PCL-Gel fibrous scaffolds containing 2 wt% MgO and preseeded with hEnSCs have nearly 79% wound closure ability while sterile gauze showed 11% of wound size reduction. Our results can be employed for biomaterials aimed at the healing of full-thickness skin wounds. © 2020 Wiley Periodicals, Inc.