Evaluation of Circulating Serum 3 Types of Microrna As Biomarkers of Oral Squamous Cell Carcinoma; a Pilot Study Publisher Pubmed



Karimi A¹ ; Bahrami N^{2, 3} ; Sayedyahossein A⁴ ; Derakhshan S⁵
Source: Journal of Oral Pathology and Medicine Published:2020

Abstract

Introduction: The microRNAs are molecules which have important biologic role and play key point in cancers. The aim of present study was to determine the miR-21, miR-24, and miR-29a expression in serum of patients with oral squamous cell carcinoma. Materials and methods: Blood samples were obtained from 40 patients (20 in cases and 20 in control group) to determine the miR-21, miR-24, and miR-29a expressions by using real-time PCR and ΔCT. Results: Mean miR-29a was −2.28 ± 2.15 and 5.61 ± 2.38 in case and control groups, respectively. The miR-21 was 6.90 ± 3.86 and −0.88 ± 2.31 in case and control groups, respectively. According to the results, miR-24 was 2.13 ± 2.89 and −0.35 ± 2.44 in case and control, respectively. A significant difference was observed on miR-21, miR-24, and miR-29a between two groups (P <.05). The results obtained by t test showed miR-21 and miR-24 were higher and miR-29a was lower in plasma of oral squamous cell carcinoma patients and this differences were significant (P <.05). Conclusion: These results suggested miR-21, miR-24, and miR-29a in serum of patients with oral squamous cell carcinoma comparing with normal group can be used as potent markers for carcinoma detection and also may be a potentially therapeutic approach in the future. More longitudinal studies with larger samples are necessary to confirm these findings. © 2019 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd