Diagnosis of Echinococcosis by Detecting Circulating Cell-Free Dna and Mirna Publisher Pubmed



Hadipour M¹ ; Fasihi Harandi M² ; Mirhendi H¹ ; Yousofi Darani H¹
Source: Expert Review of Molecular Diagnostics Published:2023

Abstract

Introduction: Diagnosis of echinococcosis is difficult and usually performed based on clinical findings, imaging, and serological test. However, all of them have limitations, especially in follow-up approaches. Areas covered: Detection of cell-free DNA (cfDNA) and micro-RNA (miRNA) is currently a hot topic for diagnosis of echinococcosis diseases. For detecting cell-free DNA in echinococcosis patient’s samples such as sera, some techniques are based on next-generation sequencing (NGS), DNA-deep sequencing, some are based on PCR-based methods, and a few works related to the detection of miRNA for the diagnosis of human echinococcosis. Expert opinion: In the detection of cell-free DNA in echinococcosis patient’ samples, NGS and DNA-deep sequencing have shown high level of sensitivity, but are not suitable for routine clinical examination as they are expensive and inaccessible in the majority of endemic areas. However, PCR-based methods have shown a sensitivity of about 20–25%. To improve the sensitivity of these tests, improving the DNA extraction method, designing appropriate primers for detecting short-length fragments of circulating DNA, using a higher volume of a serum sample, and application of more sensitive PCR methods are recommended. In the field of miRNA detection, further works are recommended. © 2023 Informa UK Limited, trading as Taylor & Francis Group.