A New Primer Pair in Its1 Region for Molecular Studies on Echinococcus Granulosus

A New Primer Pair in Its1 Region for Molecular Studies on Echinococcus Granulosus

Rahimi HR¹ ; Kia EB¹ ; Mirhendi SH¹ ; Talebi A² ; Harandi MF³ ; Jalalizand N¹ ; Rokni MB¹

Source: Iranian Journal of Public Health Published:2007

Abstract

Background: Echinocuccus granulosus, the causative agent of cystic echinococcosis has long been recognized as having a high degree of genetic divergence. The strains characterization seems to be essential for the establishment of a preventive and control strategy in every endemic area. Using DNA based methods for strain/genotype characterizations of E. granulosus have some difficulties, especially access to an efficient and pure concentration of DNA and proper primes. Methods: Using grinder method, a pure and high concentration DNA was extracted from 10 human, hydatid cysts collected from Isfahan (central Iran) hospitals, and processed for PCR reaction. Results: Using DNASIS, the primers were designed in internal transcribed spacer I (ITSI) region, following analysis of 30 E. granulosus nucleotide sequences, extracted from gene bank. Conclusion: This new and specific E. granulosus primer, which amplified DNA thoroughly can be applied for molecular studies on echinococcosis.

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Hossein Yousofi Darani (4)

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Style	Citing Format
MLA	Rahimi HR, et al.. "A New Primer Pair in Its1 Region for Molecular Studies on Echinococcus Granulosus." Iranian Journal of Public Health, vol. 36, no. 1, 2007, pp. 45-49.
APA	Rahimi HR, Kia EB, Mirhendi SH, Talebi A, Harandi MF, Jalalizand N, Rokni MB (2007). A New Primer Pair in Its1 Region for Molecular Studies on Echinococcus Granulosus. Iranian Journal of Public Health, 36(1), 45-49.
Chicago	Rahimi HR, Kia EB, Mirhendi SH, Talebi A, Harandi MF, Jalalizand N, Rokni MB. "A New Primer Pair in Its1 Region for Molecular Studies on Echinococcus Granulosus." Iranian Journal of Public Health 36, no. 1 (2007): 45-49.
Harvard	Rahimi HR et al. (2007) 'A New Primer Pair in Its1 Region for Molecular Studies on Echinococcus Granulosus', Iranian Journal of Public Health, 36(1), pp. 45-49.
Vancouver	Rahimi HR, Kia EB, Mirhendi SH, Talebi A, Harandi MF, Jalalizand N, et al.. A New Primer Pair in Its1 Region for Molecular Studies on Echinococcus Granulosus. Iranian Journal of Public Health. 2007;36(1):45-49.
BibTex	@article{ author = {Rahimi HR and Kia EB and Mirhendi SH and Talebi A and Harandi MF and Jalalizand N and Rokni MB}, title = {A New Primer Pair in Its1 Region for Molecular Studies on Echinococcus Granulosus}, journal = {Iranian Journal of Public Health}, volume = {36}, number = {1}, pages = {45-49}, year = {2007} }
RIS	TY - JOUR AU - Rahimi HR AU - Kia EB AU - Mirhendi SH AU - Talebi A AU - Harandi MF AU - Jalalizand N AU - Rokni MB TI - A New Primer Pair in Its1 Region for Molecular Studies on Echinococcus Granulosus JO - Iranian Journal of Public Health VL - 36 IS - 1 SP - 45 EP - 49 PY - 2007 ER -

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