Nanoliposomes Carrying Her2/Neu-Derived Peptide Ae36 With Cpg-Odn Exhibit Therapeutic and Prophylactic Activities in a Mice Tubo Model of Breast Cancer Publisher Pubmed



Barati N^{1, 2} ; Nikpoor AR³ ; Razazan A⁴ ; Mosaffa F^{2, 5} ; Badiee A^{1, 5} ; Arab A^{1, 2} ; Gholizadeh Z³ ; Behravan J^{2, 5} ; Jaafari MR^{1, 2, 5}
Source: Immunology Letters Published:2017

Abstract

This study was designed to prepare and characterize nanoliposomal vaccine formulation encapsulating AE36 HER2/neu-derived peptide with or without CpG and evaluate the immunologic and therapeutic responses of that in BALB/c mice model of Her2 overexpressing breast cancer. AE36 was encapsulated in liposomes composed of DOTAP, DOPE and Cholesterol (DDC) or DD with. The formulations could induce both CD8+ and CD4+ responses and stimulate production of cytokines which was detected by Enzyme-linked immunospot assay (ELISpot) kits, cytotoxicity test and intracellular cytokine assay by flow cytometry. The formulation showed both therapeutic and prophylactic effects in BALB/c mice bearing Her2+ breast cancer. DDC + CpG showed the best effect in prophylactic study and DD + pG showed the best effect in therapeutic study, which both of them decreased the size of tumors significantly. The engineered nanoliposomes containing AE36 could be a candidate vaccine for the treatment or prophylaxis of HER2+ breast cancer and merits further investigation. © 2017 European Federation of Immunological Societies