Tracking of Intraperitoneally and Direct Intrahepatic Administered Mesenchymal Stem Cells Expressing Mir-146A-5P in Mice Hepatic Tissue

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Tracking of Intraperitoneally and Direct Intrahepatic Administered Mesenchymal Stem Cells Expressing Mir-146A-5P in Mice Hepatic Tissue Publisher

Fathbayati L¹ ; Ai J² ; Mowla SJ³ ; Ebrahimibarough S² ; Motevaseli E⁴

Source: Health Biotechnology and Biopharma Published:2020

Abstract

Given the functional drawbacks in the efficacy and safety of conventional miRNA delivery approaches, we aimed to assess the fate of mesenchymal stem cells (MSCs) expressing miR-146a-5p into the mice hepatic tissue. MSCs were xeno-transplanted through direct intrahepatic and intraperitoneal routes into immunocompetent and cyclosporine A treated BALB/c mice. DNA extracts from murine organs 24 h and 28 days after xenotransplantation were analyzed by PCR method for detection of human-specific Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) sequence. Xeno-transplanted naive MSCs could not be detected after 24 h and 28 days post-infusion at the various organs of mice. Considering the miR-146a-5p expression in MSCs together with negative results of cell engraftment in hepatic tissue, the present study suggests the cell-free secretome-based miR-146a-5p delivery for modulation of altered miRNA expression in hepatic stellate cells. © 2022 by the Author(s).

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Style	Citing Format
MLA	Fathbayati L, et al.. "Tracking of Intraperitoneally and Direct Intrahepatic Administered Mesenchymal Stem Cells Expressing Mir-146A-5P in Mice Hepatic Tissue." Health Biotechnology and Biopharma, vol. 4, no. 3, 2020, pp. 56-76.
APA	Fathbayati L, Ai J, Mowla SJ, Ebrahimibarough S, Motevaseli E (2020). Tracking of Intraperitoneally and Direct Intrahepatic Administered Mesenchymal Stem Cells Expressing Mir-146A-5P in Mice Hepatic Tissue. Health Biotechnology and Biopharma, 4(3), 56-76.
Chicago	Fathbayati L, Ai J, Mowla SJ, Ebrahimibarough S, Motevaseli E. "Tracking of Intraperitoneally and Direct Intrahepatic Administered Mesenchymal Stem Cells Expressing Mir-146A-5P in Mice Hepatic Tissue." Health Biotechnology and Biopharma 4, no. 3 (2020): 56-76.
Harvard	Fathbayati L et al. (2020) 'Tracking of Intraperitoneally and Direct Intrahepatic Administered Mesenchymal Stem Cells Expressing Mir-146A-5P in Mice Hepatic Tissue', Health Biotechnology and Biopharma, 4(3), pp. 56-76.
Vancouver	Fathbayati L, Ai J, Mowla SJ, Ebrahimibarough S, Motevaseli E. Tracking of Intraperitoneally and Direct Intrahepatic Administered Mesenchymal Stem Cells Expressing Mir-146A-5P in Mice Hepatic Tissue. Health Biotechnology and Biopharma. 2020;4(3):56-76.
BibTex	@article{ author = {Fathbayati L and Ai J and Mowla SJ and Ebrahimibarough S and Motevaseli E}, title = {Tracking of Intraperitoneally and Direct Intrahepatic Administered Mesenchymal Stem Cells Expressing Mir-146A-5P in Mice Hepatic Tissue}, journal = {Health Biotechnology and Biopharma}, volume = {4}, number = {3}, pages = {56-76}, year = {2020} }
RIS	TY - JOUR AU - Fathbayati L AU - Ai J AU - Mowla SJ AU - Ebrahimibarough S AU - Motevaseli E TI - Tracking of Intraperitoneally and Direct Intrahepatic Administered Mesenchymal Stem Cells Expressing Mir-146A-5P in Mice Hepatic Tissue JO - Health Biotechnology and Biopharma VL - 4 IS - 3 SP - 56 EP - 76 PY - 2020 ER -

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