Enhanced Production and Characterization of a Highly Stable Extracellular Protease From an Extreme Halophilic Isolate Salicola Marasensis



Khoshnevis N¹ ; Rezaei S¹ ; Samaeinouroozi A¹ ; Amin M² ; Moshfegh M¹ ; Khoshayand MR² ; Faramarzi MA¹
Source: Iranian Journal of Pharmaceutical Research Published:2018

Abstract

Owing to their superior catalytic activity in the extreme conditions, extremozymes have found the potential biotechnological applications for industrial purposes. A robust extracellular protease activity was detected in the culture broth of Salicola marasensis, an extreme halophilic bacterium, after a 48 h-incubation. The effect of different media ingredients in a liquid state fermentation was followed with the aim of improving the enzyme production yield. Fractional factorial and Box-Behnken designs were applied to get a 3.4 fold (from 6.0 to 20.3 U mL−1) improvement of protease production. The distinguishing features of this enzyme were stability at a wide range of pH (5.0–11.0) and temperature (25–60 °C), significant compatibility towards organic solvents, metal ions, chemicals, and surfactants, and hydrolysis of a variety of substrates. The properties of this enzyme can be of tremendous help in terms of the halophilic proteolytic extract’s industrial applications. © 2018, Iranian Journal of Pharmaceutical Research. All rights reserved.