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Medium-Based Optimization of an Organic Solvent-Tolerant Extracellular Lipase From the Isolated Halophilic Alkalibacillus Salilacus Publisher Pubmed



Samaeinouroozi A1 ; Rezaei S1 ; Khoshnevis N1 ; Doosti M2 ; Hajihoseini R1 ; Khoshayand MR3 ; Faramarzi MA1
Authors

Source: Extremophiles Published:2015


Abstract

A haloalkaliphilic solvent-tolerant lipase was produced from Alkalibacillus salilacus within 48 h of growth in liquid medium. An overall 4.9-fold enhanced production was achieved over unoptimized media after medium optimization by statistical approaches. Plackett–Burman screening suggested lipase production maximally influenced by olive oil, KH2PO4, NaCl, and glucose; and response surface methodology predicted the appropriate levels of each parameter. Produced lipase was highly active and stable over broad ranges of temperature (15–65 °C), pH (4.0–11.0), and NaCl concentration (0–30 %) showing excellent thermostable, pH-stable, and halophilic properties. The enzyme was optimally active at pH 8.0 and 40 °C. Majority of cations, except some like Co2+ and Al3+ were positive signals for lipase activity. In addition, the presence of chemical agents and organic solvents with different log Pow was well tolerated by the enzyme. Finally, efficacy of lipase-mediated esterification of various alcohols with oleic acid in organic solvents was studied. © 2015, Springer Japan.
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