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Antimicrobial Photodynamic Therapy: Modern Technology in the Treatment of Wound Infections in Patients With Burns Publisher Pubmed



Mahmoudi H1 ; Pourhajibagher M2 ; Chiniforush N3 ; Alikhani MY1 ; Bahador A4
Authors

Source: Journal of Wound Care Published:2023


Abstract

Background: Staphylococcus aureus is one of the most frequently isolated microorganisms from burn wounds. Antimicrobial photodynamic therapy (aPDT) is a new strategy that may improve antimicrobial treatment. Method: This study evaluated three meticillin-resistant Staphylococcus aureus (MRSA) and three meticillin-sensitive Staphylococcus aureus (MSSA) clinical isolates, which produced a biofilm with 0.1mg/ml Toluidine Blue O (TBO) (Sigma-Aldrich, Germany) with an energy density of 45J/cm2 and 90J/cm2, for MRSA and MSSA, respectively. The antibiofilm potential of aPDT with TBO was analysed using crystal violet assays and scanning electron microscopy. Results: TBO-aPDT significantly degraded the biofilm formed by MRSA and MSSA clinical isolates (p < 0.05). Conclusion: Our results indicated that aPDT is an effective approach to combat bacterial biofilms associated with burn wound infection. aPDT could provide a supplemental to the treatment of wound and tissue infection, and patients with burns may benefit from combined treatments. Declaration of interest: This research was supported by Vice Chancellor for Research & Technology of Hamadan University of Medical Sciences, Hamadan, IRAN (Grant no: 960129387). The authors have no conflicts of interest to declare. 2023 MA Healthcare Ltd. © 2023 MA Healthcare Ltd. All rights reserved.
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