Tehran University of Medical Sciences

Science Communicator Platform

Stay connected! Follow us on X network (Twitter):
Share By
Molecular Typing of Clostridioides Difficile Isolates From Clinical and Non-Clinical Samples in Iran Publisher Pubmed



Kouhsari E1, 2 ; Douraghi M3 ; Fakhre Yaseri H4 ; Talebi M1 ; Ahmadi A2 ; Sholeh M1 ; Amirmozafari N1
Authors

Source: APMIS Published:2019


Abstract

Clostridioides difficile is a major cause of nosocomial infectious diarrhea in hospitalized patients throughout the world. We aimed to characterize C. difficile isolates among hospitalized patients, hospital staffs, and hospital environment samples obtained in three tertiary care hospitals of Iran with regard to their molecular types between June 2016 and November 2017. The toxigenicity of C. difficile isolates was determined by toxigenic culture and multiplex-PCR. Toxigenic C. difficile isolates collected were ribotyped using capillary gel electrophoresis-based PCR and the database of WEBRIBO (http://webribo.ages.at). Of 500 clinical and non-clinical samples, toxigenic C. difficile were identified in 35 of 250 stool samples (14%) and in 3 of 250 swabs (1.2%). The most frequently found ribotypes (RTs) were 039, AI-12, and AI-21 (15.8, 10.52, and 10.52% of all isolates, respectively). Further RTs were: 017, 001, AI-3, AI-15, AI-18, AI-10, AI-4, and PR21195 (as new ribotype). The epidemic RTs (027 and 078) seen in the Europe, North America, and Asia were completely absent in this study. © 2019 APMIS. Published by John Wiley & Sons Ltd
Related Docs
View other Related Docs
1. The Emergence of Metronidazole and Vancomycin Reduced Susceptibility in Clostridium Difficile Isolates in Iran, Journal of Global Antimicrobial Resistance (2019)
2. High Prevalence of Clostridiodes Diffiicle Pcr Ribotypes 001 and 126 in Iran, Scientific Reports (2020)
3. Rapid Simultaneous Molecular Stool-Based Detection of Toxigenic Clostridioides Difficile by Quantitative Taqman Real-Time Pcr Assay, Clinical Laboratory (2019)
Experts (# of related papers)
View all Related Experts
Masoumeh Douraghi (8)
Mohammad Vasei (2)
Other Related Docs
4. Characterization of Clostridioides Difficile Isolates Recovered From Hospitalized Patients and the Hospitals Environment and Air: A Multicenter Study, Anaerobe (2019)
5. Pathogenicity Locus Determinants and Toxinotyping of Clostridioides Difficile Isolates Recovered From Iranian Patients, New Microbes and New Infections (2018)
6. Highly Antibiotic-Resistant Clostridium Difficile Isolates From Iranian Patients, Journal of Applied Microbiology (2018)
7. Isolation and Characterization of a Multidrug-Resistant Clostridioides Difficile Toxinotype V From Municipal Wastewater Treatment Plant, Journal of Environmental Health Science and Engineering (2020)
8. Polymorphisms in the Genes Encoding Surface Associated Proteins of Clostridioides Difficile Isolates, Infection# Genetics and Evolution (2020)
9. Antibiotic-Associated Diarrhea Mediated by Toxigenic Clostridioides Difficile Infection in Iran: A Clinical Survey by Multiplex Real-Time Polymerase Chain Reaction and Toxigenic Culture, Govaresh (2022)
10. Intestinal Colonization by Campylobacter Jejuni, Clostridium Difficile, and Clostridium Perfringens Among Commensal Rattus Norvegicus in the Urban Areas of Tehran, Iran, BioMed Research International (2024)
11. The Prevalence of Clostridium Difficile and Clostridium Perfringens in Minced and Ground Beef in Iran, Journal of Chemical Health Risks (2022)
12. Toxigenic Clostridium Difficile-Mediated Diarrhoea in Hematopoietic Stem Cell Transplantation In-Patients: Rapid Diagnosis and Efficient Treatment, Epidemiology and Infection (2021)
13. Enterococcus Faecium From Meat, Chicken and Cheese, Jundishapur Journal of Microbiology (2015)
14. A Two-Step Approach for Diagnosing Glutamate Dehydrogenase Genes by Conventional Polymerase Chain Reaction From Clostridium Difficile Isolates, Middle East Journal of Digestive Diseases (2019)
15. Significant Presence of Clostridioides Difficile in Colorectal Cancer Patients by Taqman Real-Time Pcr, Iranian Journal of Medical Microbiology (2024)
16. Clinical and Pathological Features of Ulcerative Colitis in Patients With and Without Clostridium Difficile Infection; an Observational Study, Middle East Journal of Digestive Diseases (2019)
17. Subtractive Genomic Approach Toward Introduction of Novel Immunogenic Targets Against Clostridioides Difficile: Thinking Out of the Box, Microbial Pathogenesis (2022)
18. The Rate and Importance of Clostridium Difficile in Colorectal Cancer Patients, Gastroenterology and Hepatology from Bed to Bench (2019)
19. Anti-Tnf Containing Regimens May Be Associated With Increased Risk of Clostridioides Difficile Infection in Patients With Underlying Inflammatory Bowel Disease, Current Research in Translational Medicine (2020)
20. Antimicrobial Susceptibility Patterns of Enteroaggregative E. Coli, As the Most Common Diarrheagenic E. Coli, Associated to Gastroenteritis Outbreaks in Iran, Archives of Pediatric Infectious Diseases (2018)