Study of Third-Degree Burn Wounds Debridement and Treatment by Actinidin Enzyme Immobilized on Electrospun Chitosan/Peo Nanofibers in Rats Publisher



Ketabchi N¹ ; Dinarvand R² ; Adabi M¹ ; Gholami M³ ; Firoozi S¹ ; Amanzadi B¹ ; Faridimajidi R¹
Source: Biointerface Research in Applied Chemistry Published:2021

Abstract

In this study, the actinidin enzyme that was extracted from kiwi fruit was immobilized on a scaffold based on electrospun chitosan nanofibers, and its ability to treat burn wounds was investigated. Afterward, cytotoxicity of the nanofibrous patch and nanofibers with actinidin enzyme was evaluated by MTT test separately on skin fibroblast cells (AGO1522), and their efficacy was investigated on rat model’s full-thickness burn wounds. As a result, SEM showed the diameters of the fibers that were in a range of 100-200 nm depending on the electrospinning condition. MTT results confirmed the cell viability is exposing the enzyme and nanofibers. Then evaluating wound closure percentage in animal samples was analyzed by statistical tests. Considering P<0/05, a significant difference in the rate of wound closure between the groups was observed. Histopathological assays also showed the accelerating effect of the nanofibrous patch with an enzyme in epithelisation, angiogenesis, collagen deposition, and its effective role in scar formation prevention. In this study, actinidin also showed perfect compatibility with cells, and chitosan could prevent wounds infection. So using this bandage could eliminate the scars and reduce the treatment time. © 2020 by the authors.