Α-Glucosidase Inhibitors From Marrubium Astracanicum: Isolation and Molecular Docking Publisher



Kazemi R¹ ; Delnavazi MR¹ ; Parsakhankandi H² ; Mojtabavi S³ ; Hoseinsalari A¹ ; Faramarzi MA³ ; Khanavi M^{1, 4}
Source: Revista Brasileira de Farmacognosia Published:2022

Abstract

Marrubium astracanicum Jacq., Lamiaceae, is an herbaceous plant distributed in Iran, Turkey, and southern Caucasia. In the present study, α-glucosidase inhibitory principles of M. astracanicum aerial parts were investigated by a bioassay-guided approach. The extraction procedure from the plant aerial parts was carried out successively using petroleum ether, ethyl acetate, and methanol. The ethyl acetate extract, with the highest in vitro α-glucosidase inhibitory activity, was subjected to further chemical investigation. The structures of the isolated compounds were elucidated by 1H-NMR and 13C-NMR spectral analyses as apigenin-7-O-(3′′-(E)-p-coumaroyl)-β-d-glucoside, apigenin-7-O-(6′′-(E)-p-coumaroyl)-β-d-glucoside, martynoside, apigenin-7-O-β-d-glucoside, and verbascoside. In addition, the antidiabetic potential of the isolated compounds was evaluated in an in vitro α-glucosidase inhibitory assay, and kinetic and molecular docking studies were done for the most active compound. Apigenin-7-O-(3′′-(E)-p-coumaroyl)-β-d-glucoside and apigenin-7-O-(6′′-(E)-p-coumaroyl)-β-d-glucoside showed the most potent in vitro α-glucosidase inhibitory activity with IC50 values of 57.4 ± 1.2 and 186.4 ± 2.5 μM, respectively, which was about 4 to 13 times lower than the IC50 value of the reference compound acarbose (751.2 ± 0.4 μM). Graphical abstract: [Figure not available: see fulltext.]. © 2022, The Author(s) under exclusive licence to Sociedade Brasileira de Farmacognosia.