In Vitro Antimicrobial and Acetylcholinesterase Inhibitory Activities of Coumarins From Ferulago Carduchorum Publisher



Golfakhrabadi F^{1, 2} ; Shams Ardakani MR² ; Saeidnia S³ ; Akbarzadeh T⁴ ; Yousefbeyk F⁵ ; Jamalifar H⁶ ; Khanavi M^{2, 7}
Source: Medicinal Chemistry Research Published:2016

Abstract

Ferulagocarduchorum (Apiaceae) is an endemic plant of Iran. From the hexane and ethyl acetate extracts of F. carduchorum seven coumarins, one flavonoid and one steroid were isolated using column chromatography with silica gel and Sephadex LH20 as the stationary phases. Antimicrobial activity of the isolated compounds was examined by a broth microdilution method. Acetylcholinesterase (AChE) inhibitory activity of isolated coumarins was also investigated. The isolated compounds were identified as suberosin, suberenol, bergapten, xanthotoxin, isopimpinellin, prantschimgin, β-sitosterol and hesperetin by comparison of their NMR and MS spectral data with those reported in the literature. Evaluation of the minimum inhibitory concentration (MIC) for each compound showed that hesperetin (flavonoid) was the most potent antimicrobial agent against a gram-positive bacterium (Staphylococcusaureus) and among the coumarins, bergapten had the best activity against S. aureus and Candidaalbicans. All coumarins inhibited AchE enzyme, in which xanthotoxin showed the most inhibitory among them (IC50 = 39.64 µM). Our results indicate that isolated coumarins are effective against the tested bacterial strains and have AchE inhibitory activity suggesting their potential for commercial applications. © 2016, Springer Science+Business Media New York.