Cytotoxic Impact of Naringenin-Loaded Solid Lipid Nanoparticles on Rin5f Pancreatic Β Cells Via Autophagy Blockage Publisher Pubmed



P Mohammadi Pour PARDIS ; Z Nouri ZEINAB ; D Ghasemi DARIUSH ; S Sajadimajd SORAYA ; Mh Farzaei Mohammad HOSSEIN
Source: Recent Advances in Drug Delivery and Formulation Published:2024

Abstract

Background: Autophagy plays a crucial role in modulating the proliferation of cancer diseases. However, the application of Naringenin (Nar), a compound with potential benefits against these diseases, has been limited due to its poor solubility and bioavailability. Objective: This study aimed to develop solid lipid nanoparticles (Nar-SLNs) loaded with Nar to enhance their therapeutic impact. Methods: In vitro experiments using Rin-5F cells exposed to Nar and Nar-SLNs were carried out to investigate the protective effects of Nar and its nanoformulation against the pancreatic cancer cell line of Rin-5F. Results: Treatment with Nar and Nar-SLN led to an increase in autophagic markers (Akt, LC3, Beclin1, and ATG genes) and a decrease in the level of miR-21. Both Nar and Nar-SLN treatments inhibited cell proliferation and reduced the expression of autophagic markers. Notably, Nar-SLNs exhibited greater efficacy compared to free Nar. Conclusion: These findings suggest that SLNs effectively enhance the cytotoxic impact of Nar, making Nar-SLNs a promising candidate for suppressing or preventing Rin-5F cell growth. © 2024 Elsevier B.V., All rights reserved.