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Protective Effect of Alginate Extraction of Brown Algae on Micrornas in Type 1 Diabetes of Rats Publisher



Sharifzadeh M1 ; Esmaeilibandboni A2 ; Emami M1 ; Mirshafiey A3, 4 ; Javanbakht MH5
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Source: International Journal of Nutrition Sciences Published:2024


Abstract

Background: MicroRNAs expressions that are known as Post-transcriptional repressors of gene expression in animals and plants have an important role in diabetic consequences and pathogenesis. This research aimed to investigate the expression of miR-34a, miR-126, and miR-125a-5p levels for diagnosis of diabetes, and the effect of α-L-guluronic acid and β-D-mannuronic acid supplementation on expression patterns of these MicroRNAs in diabetic rats. Methods: Total MicroRNAs levels were measured by Quantitative reverse transcription polymerase chain reaction (qRT-PCR) method. Body weight, fasting insulin level, food intake and Vitamin D level in four groups were also assessed after 4 weeks. Results: The analyses illustrate marked declines in expression of miR-126 (p=0.009) and miR-125a-5p (p=0.019) levels in the control diabetic group when compared to the control healthy group. Expression levels of miR-126 (p=0.026) and miR-125a-5p (p=0.041) were upregulated considerably in diabetic rats after treatment with β-D-mannuronic acid and α-L-guluronic acid, respectively. Conclusion: These results suggest that use of miR-126 and 125a-5p is a sensible criterion for diagnosing diabetes. β-D-mannuronic acid is also considered a new therapeutic agent to correct disturbances in the MicroRNAs profile of diabetic rats. © International Journal of Nutrition Sciences.
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