The Effects of All Trans Retinoic Acid, Vitamin D3 and Their Combination on Plasma Levels of Mirna-125A-5P, Mirna-34A, and Mirna-126 in an Experimental Model of Diabetes Publisher



Sharifzadeh M¹ ; Esmaeilibandboni A^{2, 3} ; Emami MR¹ ; Naeini F¹ ; Zarezadeh M^{4, 5} ; Javanbakht MH⁶
Source: Avicenna Journal of Phytomedicine Published:2022

Abstract

Objective: The purpose of this study was to evaluate the effects of ATRA (all trans retinoic acid), vitamin D3, and their combination on circulating levels of miR (MicroRNA) -125a-5p, miR-126, and miR-34ain diabetic rats. Materials and Methods: Total miRNA was extracted from plasma samples. miRNA expression profiles of 30 rats in five groups were analyzed after 4-week intervention. The expression levels of miRNAs were measured using qRT-PCR. Results: We analyzed the expression of miR-126, miR-125a-5p, and miR-34a in serum among all five groups (p=0.268). The levels of miRNA-126 (p=0.004) and miR-125a-5p (p=0.014) showed a significant difference among our experimental groups. The circulating levels of miR-126 decreased in DC (Diabetic control) group compared to the HC (Healthy control) group (p=0.009). In addition, vitamin D3+ATRA supplementation increased miR-126 expression (p=0.014). Moreover, the levels of miR-125a-5p decreased in the DC group compared to the HC group (p=0.019). Conclusion: The expression of miR-126 and miR-125a-5p decreased in diabetic rats. Also, vitamin D3+ATRA can be considered a new therapeutic agent that can elevate miR-126 expression and prevent diabetes-related cardiovascular complications. © 2022 Mashhad University of Medical Sciences. All rights reserved.