Co-Loading of Cisplatin and Methotrexate in Nanoparticle-Based Pcl-Peg System Enhances Lung Cancer Chemotherapy Effects Publisher



Akbari E^{1, 6} ; Mousazadeh H¹ ; Hanifehpour Y² ; Mostafavi E³ ; Gorabi AM¹⁰ ; Nejati K⁸ ; Keyhanvar P⁴ ; Pazokitoroudi H⁹ ; Mohammadhosseini M⁷ ; Akbarzadeh A^{4, 5}
Source: Journal of Cluster Science Published:2022

Abstract

Abstract: Since the anticancer drugs exhibited a variety of inhibitory mechanisms in cancer cells, the use of two or more anticancer drugs may have excellent therapeutic effects, particularly in drug-resistant tumors. In this study, the efficient entrapment of two clinically used single-agent drugs, Cisplatin (CDDP) and Methotrexate (MTX) is reported against lung cancer cell lines. Biodegradable polymeric nanoparticles perform to be a favorable environment-responsive controlled drug release system. MTX@CDDP were simultaneously encapsulated into the biodegradable poly (ε-caprolactone) (PCL) modified poly (ethylene glycol) (PEG) copolymer. The spherical nanoparticle was identified via scanning electron microscopy (SEM). Additionally, the antitumor activity and apoptosis induction of designed duel drug-loaded vectors were assessed against A549 cell lines by qRT-PCR, MTT assay, and DAPI staining. The nanoformulation loaded with MTX@CDDP statistically reduced the cell activity of A549. The results indicate that MTX@CDDP-loaded PCL-PEG nanoparticles can be further utilized for treating non-small-cell lung cancer as a promising therapeutic approach. Graphical Abstract: [Figure not available: see fulltext.]. © 2021, The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.