Evaluation of Relative Frequency of Single Nucleotide Polymorphism G870a (Rs9344) in Cyclind1 Gene in Colorectal Cancer Patients and Healthy Subjects of Isfahan Province, Iran



Rahimirad S¹ ; Salehi R² ; Saffar B¹ ; Niaee M¹
Source: Journal of Isfahan Medical School Published:2016

Abstract

Background: Colorectal cancer (CRC) is one of the most common cancers in the world. High activity of CyclinD1 gene has been seen in the progress of this cancer. A common polymorphism (G870A) in exon 4 of CyclinD1 produces a variant transcript with longer half-life and may cause uncontrollable cellular growth thus contributing to cancer development. This study was performed to evaluate the frequency of CCND1 G870A polymorphism between CRC cases and controls. Methods: DNA samples from peripheral blood leukocytes of 50 patients with sporadic colorectal cancer and 50 healthy subjects were extracted. CCND1 G870A polymorphism was genotyped by polymerase chain reaction -restriction fragment length polymorphism (PCR-RFLP) in healthy subjects and patients. Statistical analysis was performed by chi-squared test via SPSS software. Findings: Relationship between CCND1 G870A polymorphism in allele frequencies between cases and controls were not observed (P = 0.204). While the frequency of AA genotype was significantly higher in patients (P = 0.040, 95% CI = 1.13-5.54, OR = 2.25). Conclusion: According to the significant association observed between certain genotypes of this locus (AA) with colorectal cancer, it can be used in the future as a good prognostic marker in CRC screening programs. © 2016, Isfahan University of Medical Sciences(IUMS). All rights reserved.