Generation of Insulin-Producing Cells From Human Induced Pluripotent Stem Cells on Plla/Pva Nanofiber Scaffold Publisher Pubmed



Enderami SE^{1, 2} ; Kehtari M³ ; Abazari MF⁴ ; Ghoraeian P⁴ ; Nouri Aleagha M⁴ ; Soleimanifar F⁵ ; Soleimani M⁶ ; Mortazavi Y^{2, 7} ; Nadri S² ; Mostafavi H⁸ ; Askari H⁹
Source: Artificial Cells# Nanomedicine and Biotechnology Published:2018

Abstract

Pancreatic tissue engineering as a therapeutic option for restoring and maintenance of damaged pancreas function has a special focus to using synthetic Scaffolds. This study was designed to evaluate pancreatic differentiation of human induced pluripotent stem cells (hiPSCs) on poly-L-lactic acid and polyvinyl alcohol (PLLA/PVA) scaffolds as 3 D matrix. During differentiation process, morphology of cells gradually changed and iPSCs derived insulin producing cells (iPSCs-IPCs) formed spherical shaped cell aggregation that was the typical shape of islets of pancreas. The highly efficient differentiation of iPSCs into a relatively homogeneous population of IPCs was shown by immunostaining. Real-time reverse transcription polymerase chain reaction (RT-PCR) results demonstrated that iPSCs-IPCs expressed pancreas-specific transcription factors (Pdx1, insulin, glucagon and Ngn3). The expressions of these transcription factors in PLLA/PVA scaffold were significantly higher than 2 D groups. Furthermore, we showed that concentration of insulin and C-peptide in PLLA/PVA scaffold and/or 2 D culture in response to various concentrations of glucose increased but the difference between them were not significant. Altogether the current results demonstrated that PLLA/PVA scaffold could provide the microenvironment that promotes the pancreatic differentiation of iPSCs, up-regulate pancreatic-specific transcription factors and improved metabolic activity. © 2018, © 2018 Informa UK Limited, trading as Taylor & Francis Group.