Tehran University of Medical Sciences

Science Communicator Platform

Stay connected! Follow us on X network (Twitter):
Share By
Comparison of Human-Induced Pluripotent Stem Cells and Mesenchymal Stem Cell Differentiation Potential to Insulin Producing Cells in 2D and 3D Culture Systems in Vitro Publisher Pubmed



Abazari MF1 ; Nasiri N2 ; Nejati F2 ; Zare Karizi S3 ; Amini Faskhodi M4 ; Saburi E5 ; Aghapur N6 ; Mahdavi MR7 ; Ardeshirylajimi A8 ; Enderami SE9, 10 ; Soleimanifar F11, 12
Authors

Source: Journal of Cellular Physiology Published:2020


Abstract

Diabetes is one of the most common diseases in the world that is chronic, progressive, and costly, and causes many complications. Common drug therapies are not able to cure it, and pancreas transplantation is not responsive to the high number of patients. The production of the insulin producing cells (IPCs) from the stem cells in the laboratory and their transplantation to the patient's body is one of the most promising new approaches. In this study, the differentiation potential of the induced pluripotent stem cells (iPSCs) and mesenchymal stem cells (MSCs) into IPCs was compared to each other while cultured on poly(lactic-co-glycolic) acid (PLGA)/polyethylene glycol (PEG) nanofibrous scaffold as a 3D substrate and tissue culture polystyrene (TCPS) as a 2D substrate. Although the expression level of the insulin, Glut2 and pdx-1 genes in stem cells cultured on 3D substrate was significantly higher than the stem cells cultured on 2D substrate, the highest expression level of these genes was detected in the iPSCs cultured on PLGA-PEG. Insulin and C-peptide secretions from differentiated cells were also investigated and the results showed that secretions in cultured iPSCs on the PLGA-PEG were significantly higher than cultured iPSCs on the TCPS and cultured MSCs on both PLGA-PEG and TCPS. In addition, insulin protein was also expressed in the cultured iPSCs on the PLGA-PEG significantly higher than cultured MSCs on the PLGA-PEG. It can be concluded that differentiation potential of iPSCs into IPCs is significantly higher than human MSCs at both 2D and 3D culture systems. © 2019 Wiley Periodicals, Inc.
Related Docs
View other Related Docs
1. Pcl/Pva Nanofibrous Scaffold Improve Insulin-Producing Cells Generation From Human Induced Pluripotent Stem Cells, Gene (2018)
2. Generation of High Yield Insulin-Producing Cells (Ipcs) From Various Sources of Stem Cells, Vitamins and Hormones (2021)
3. Three-Dimensional Nanofiberous Plla/Pcl Scaffold Improved Biochemical and Molecular Markers Hips Cell-Derived Insulin-Producing Islet-Like Cells, Artificial Cells# Nanomedicine and Biotechnology (2018)
Experts (# of related papers)
View all Related Experts
Ghodratollah Panahi (1)
Moloud Absalan (1)
Other Related Docs
4. Generation of Insulin-Producing Cells From Human Induced Pluripotent Stem Cells on Plla/Pva Nanofiber Scaffold, Artificial Cells# Nanomedicine and Biotechnology (2018)
5. Collagen Coated Electrospun Polyethersulfon Nanofibers Improved Insulin Producing Cells Differentiation Potential of Human Induced Pluripotent Stem Cells, Artificial Cells# Nanomedicine and Biotechnology (2018)
6. A Novel Silk/Pes Hybrid Nanofibrous Scaffold Promotes the in Vitro Proliferation and Differentiation of Adipose-Derived Mesenchymal Stem Cells Into Insulin Producing Cells, Polymers for Advanced Technologies (2020)
7. Generation of High-Yield Insulin Producing Cells From Human-Induced Pluripotent Stem Cells on Polyethersulfone Nanofibrous Scaffold, Artificial Cells# Nanomedicine and Biotechnology (2018)
8. Phbv Nanofibers Promotes Insulin-Producing Cells Differentiation of Human Induced Pluripotent Stem Cells, Gene (2021)
9. The Role of Micrornas in the Induction of Pancreatic Differentiation, Current Stem Cell Research and Therapy (2020)
10. Platelet-Rich Plasma Incorporated Electrospun Pva-Chitosan-Ha Nanofibers Accelerates Osteogenic Differentiation and Bone Reconstruction, Gene (2019)
11. The Generation of Islet-Like Insulin-Producing Cells From Wharton's Jelly-Derived Mesenchymal Stem Cells on the Pes/Fish Gelatin Scaffold, Regenerative Therapy (2024)
12. Improved Osteogenic Differentiation of Human Induced Pluripotent Stem Cells Cultured on Polyvinylidene Fluoride/Collagen/Platelet-Rich Plasma Composite Nanofibers, Journal of Cellular Physiology (2020)
13. Molecular Study of the Proliferation Process of Beta Cells Derived From Pluripotent Stem Cells, Molecular Biology Reports (2022)
14. Nanofiber-Based Systems Intended for Diabetes, Journal of Nanobiotechnology (2021)
15. Bladder Smooth Muscle Cell Differentiation of the Human Induced Pluripotent Stem Cells on Electrospun Poly(Lactide-Co-Glycolide) Nanofibrous Structure, Gene (2019)
16. Improvement of Hepatogenic Differentiation of Ips Cells on an Aligned Polyethersulfone Compared to Random Nanofibers, Artificial Cells# Nanomedicine and Biotechnology (2018)
17. Induced Pluripotent Stem Cells As a New Getaway for Bone Tissue Engineering: A Systematic Review, Cell Proliferation (2017)
18. Comparison of Insulin Secretion by Transduced Adipose-Derived and Endometrial-Derived Stem Cells in 2D and 3D Cultures on Fibrin Scaffold, Journal of Biomedical Materials Research - Part A (2021)
19. Trimetazidine Preconditioning Potentiates the Effect of Mesenchymal Stem Cells Secretome on the Preservation of Rat Pancreatic Islet Survival and Function in Vitro, Applied Biochemistry and Biotechnology (2023)
20. In Vitro Osteogenic Differentiation Potential of the Human Induced Pluripotent Stem Cells Augments When Grown on Graphene Oxide-Modified Nanofibers, Gene (2019)