Granulocyte-Macrophage Colony-Stimulating Factor, a Potent Adjuvant for Polarization to Th-17 Pattern: An Experience on Hiv-1 Vaccine Model Publisher Pubmed



Mahdavi M¹ ; Tajik AH² ; Ebtekar M³ ; Rahimi R³ ; Adibzadeh MM⁴ ; Moozarmpour HR⁵ ; Beikverdi MS⁶ ; Olfat S⁷ ; Hassan ZM³ ; Choopani M⁸ ; Kameli M⁸ ; Hartoonian C⁹
Source: APMIS Published:2017

Abstract

Cytokines are mediators for polarization of immune response in vaccines. Studies show that co-immunization of DNA vaccines with granulocyte-macrophage colony-stimulating factor (GM-CSF) can increase immune responses. Here, experimental mice were immunized with HIV-1tat/pol/gag/env DNA vaccine with GM-CSF and boosted with recombinant vaccine. Lymphocyte proliferation with Brdu and CTL activity, IL-4, IFN-γ, IL-17 cytokines, total antibody, and IgG1 and IgG2a isotypes were assessed with ELISA. Results show that GM-CSF as adjuvant in DNA immunization significantly increased lymphocyte proliferation and IFN-γ cytokines, but CTL response was tiny increased. Also GM-CSF as adjuvant decreased IL-4 cytokine vs mere vaccine group. IL-17 in the group that immunized with mixture of DNA vaccine/GM-CSF was significantly increased vs DNA vaccine group. Result of total antibody shows that GM-CSF increased antibody response in which both IgG1 and IgG2a increased. Overall, results confirmed the beneficial effect of GM-CSF as adjuvant to increase vaccine immunogenicity. The hallmark result of this study was to increase IL-17 cytokine with DNA vaccine/GM-CSF immunized group. This study for the first time provides the evidence of the potency of GM-CSF in the induction of IL-17 in response to a vaccine, which is important for control of infection such as HIV-1. © 2017 APMIS. Published by John Wiley & Sons Ltd