Pseudomonas Aeruginosa Flagellin As an Adjuvant: Superiority of a Conjugated Form of Flagellin Versus a Mixture With a Human Immunodeficiency Virus Type 1 Vaccine Candidate in the Induction of Immune Responses Publisher Pubmed



Delavari S¹ ; Sohrabi M¹ ; Ardestani MS² ; Faezi S³ ; Tebianian M⁴ ; Taghizadeh M⁵ ; Shajiei A⁶ ; Hosseini SY⁷ ; Moghaddampour M^{5, 8} ; Mahdavi M⁹
Source: Journal of Medical Microbiology Published:2015

Abstract

In the present study, the adjuvant activity of flagellin was compared, in the conjugated and mixed forms, against a peptide vaccine from human immunodeficiency virus type 1 (HIV-1) p24–Nef vaccine candidate. Mice were immunized with the HIV-1 p24–Nef peptide with flagellin in both conjugated and mixed forms. Lymphocyte proliferation, CTL activity, and IL-4 and IFN-γ cytokines were evaluated by bromodeoxyuridine, carboxyfluorescein succinimidyl ester and ELISA methods, respectively. At the same time, the frequency of IFN-γ-producing T-lymphocytes, as well as total and isotype-specific antibodies, were assessed by ELISPOT and indirect ELISA, respectively. Our experimental results showed that the immunized mice with the HIV-1 p24–Nef conjugated or mixed forms of flagellin led to increases in the proliferative responses and Th1 cytokine pattern. The conjugated form of vaccine led to increased CTL activity and a Th1 cytokine pattern of immune responses, as well as an IgM isotype of humoral responses in comparison with the mixed form. The flagellin-conjugated vaccine seems to be more potent in increasing vaccine immunogenicity. © 2015 The Authors.