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Effect of Neohesperidin Dihydrochalcone on the Activity and Stability of Alpha-Amylase: A Comparative Study on Bacterial, Fungal, and Mammalian Enzymes Publisher Pubmed



Kashaniamin E1, 2 ; Ebrahimhabibi A1, 2 ; Larijani B2 ; Moosavimovahedi AA3
Authors

Source: Journal of Molecular Recognition Published:2015


Abstract

Neohesperidin dihydrochalcone (NHDC) was recently introduced as an activator of mammalian alpha-amylase. In the current study, the effect of NHDC has been investigated on bacterial and fungal alpha-amylases. Enzyme assays and kinetic analysis demonstrated the capability of NHDC to significantly activate both tested alpha-amylases. The ligand activation pattern was found to be more similar between the fungal and mammalian enzyme in comparison with the bacterial one. Further, thermostability experiments indicated a stability increase in the presence of NHDC for the bacterial enzyme. In silico (docking) test locates a putative binding site for NHDC on alpha-amylase surface in domain B. This domain shows differences in various alpha-amylase types, and the different behavior of the ligand toward the studied enzymes may be attributed to this fact. Copyright © 2015 John Wiley & Sons, Ltd.