Comprehensive Study of Serratia Peptidase Immobilization From Serratia Sp. Zf03 Onto Chitosan Nanogels; [Serratia Sp. Zf03 Kaynakli Serratia Peptidaz’In Kitosan Nanojellerin Uzerine Immobilziasyonunun Kapsamli Incelenmesi] Publisher



Salarizadeh N² ; Hasannia S¹ ; Sajedi RH³ ; Lamei N⁴ ; Mohsenifar A⁵ ; Noghabi KA⁵
Source: Turkish Journal of Biochemistry Published:2016

Abstract

Objective: In the present work, we have extended the study and immobilized the metalloprotease enzyme in glutaraldehyde cross-linked chitosan nanogels to scrutinize the enzyme’s features including stability over its soluble free form. Method: The immobilized metalloprotease was character ized using scanning electron microscopy (SEM), followed by Fourier transform infrared (FTIR) spectroscopy. The enzyme is optimally active at 50°C and pH range of 8.0-10. Results: Thermal stability of the enzyme enhanced when immobilized on the nanogel. After 5 min of incubation at 50°C, immobilized enzymes retained 60% of their original activity, while negligible activity (23%) was observed in the case of the free enzyme. Conclusion: The results obtained here provide a powerful demonstration of the benefits of taking the glutaraldehyde cross-linked chitosan matrices to enhance metallo protease stability. The high stability of the immobilized enzyme serves to improve its performance for possible application on the industrial scale. © 2016 Turkish Biochemistry Society. All rights reserved.