Investigation of Epstein–Barr Virus, Cytomegalovirus, Human Herpesvirus 6, and Polyoma Viruses (Jc Virus, Bk Virus) Among Gastric Cancer Patients: A Cross Sectional Study Publisher



Soltani S^{1, 2} ; Farahani A^{3, 4} ; Shahbahrami R¹ ; Shateri Z⁵ ; Emadi MS⁶ ; Pakzad R^{7, 8} ; Lotfi M⁹ ; Asanjarani B¹⁰ ; Rasti A¹¹ ; Erfani Y⁶ ; Siri G¹²
Source: Health Science Reports Published:2024

Abstract

Background and Aims: Gastric cancer is a significant global issue with a high death rate. This malignancy could be associated with several viral agents such as EBV, CMV, HHV-6, JCV, and BKV. Objective: Evaluation of EBV, CMV, HHV-6, and JCV, BKV frequency among gastric cancer patients. Methods: In this cross-sectional study, a total number of 60 gastric cancer specimens (32 male, 28 female) were retrieved from the pathology lab. Formalin-fixed paraffin-embedded tissue was used for molecular testing. DNA was extracted from samples, according to protocol, and used for PCR reaction. Polymerase chain reactions were used to assess CMV, EBV, HHV-6, JCV, and BKV frequency. Results and Conclusion: The mean age of the participants was 61 years and 53.3% (32) of the participants were Male. A total number of 5 samples (8.34%) were infected with viral agents. Four male gastric samples were infected with EBV (6.67%) and only one female sample contained the BKV genome (1.67%). Totally 8.34% of the samples were infected with EBV and BKV. The CMV, HHV-6, and JCV genome was not detected in the samples. In conclusion, the presence of two viral agents including EBV and BKV among male and female samples respectively, and the genome of other viruses were not detected. © 2024 The Authors. Health Science Reports published by Wiley Periodicals LLC.