Fluorescence Based Turn-On Strategy for Determination of Microrna-155 Using Dna-Templated Copper Nanoclusters Publisher



Borghei YS¹ ; Hosseini M^{1, 2} ; Ganjali MR^{3, 4}
Source: Microchimica Acta Published:2017

Abstract

The authors report on a new approach for the determination of the breast cancer biomarker microRNA-155 (miRNA-155). It is based on the measurement of the fluorescence shift of oligonucleotide-templated copper nanoclusters (DNA-CuNC). A probe DNA was designed that acts as a template for the preparation of CuNC which, under 400 nm excitation, exhibit strong fluorescence enhancement at 490 nm and a 90 nm Stokes shift after binding to target miRNA-155 and formation of a DNA-RNA heteroduplex. Under the optimal conditions, the fluorescence of the DNA-CuNC increases with increasing concentration of miRNA-155 in the range from 50 pM to 10 nM, with a 11 pM detection limit. The assay has excellent selectivity over noncomplementary RNA. The method was applied to the determination of miRNA-155 in the presence of human plasma and saliva. [Figure not available: see fulltext.]. © 2017, Springer-Verlag Wien.