Profiling Inflammatory Mechanisms, Hyperphosphorylated Tau of Hippocampal Tissue and Spatial Memory Following Vitamin D3 Treatment in the Mice Model of Vascular Dementia Publisher Pubmed



Sadeghzadeh J¹ ; Jafarzadeh J^{2, 3} ; Hadinezhad P^{4, 5} ; Nazari A⁶ ; Sohrabi S⁷ ; Musazadeh V^{2, 8} ; Barzegar A³ ; Shahabi P^{9, 10}
Source: International Immunopharmacology Published:2023

Abstract

Background: The aim of this study was to investigate the effect of vitamin D3 (VitD3) on inflammatory mechanisms, hyperphosphorylated tau (p-tau) in the hippocampus, and cognitive impairment of the mouse model of vascular dementia (VaD). Methods: In this study, 32 male mice were randomly assigned to the control, VaD, VitD3 (300 IU/Kg/day), and VitD3 (500 IU/Kg/day) groups. VaD and VitD3 groups were gavaged daily for 4 weeks with a gastric needle. For biochemical assessments, blood samples and the hippocampus were isolated. IL-1β and TNF-α were analyzed by ELISA, and p-tau and other inflammatory molecules were measured by western blot. Results: VitD3 supplements significantly (P < 0.05) decreased the level of inflammatory factors in the hippocampus and prevented apoptosis. However, regarding p-tau in hippocampal tissue, this decrease was not statistically significant (P > 0.05). The results of behavioral assessments showed that VitD3 significantly improved the spatial memory of treated mice. Conclusion: These results suggest that the neuroprotective effects of VitD3 are mainly associated with their anti-inflammatory effects. © 2023 Elsevier B.V.