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Effects of Sub-Lethal Doses of Photo-Activated Disinfection Against Porphyromonas Gingivalis for Pharmaceutical Treatment of Periodontal-Endodontic Lesions Publisher Pubmed



Pourhajibagher M1, 2, 3 ; Chiniforush N2 ; Raoofian R4, 5 ; Ghorbanzadeh R6 ; Shahabi S7 ; Bahador A1, 2, 3
Authors

Source: Photodiagnosis and Photodynamic Therapy Published:2016


Abstract

Background Microorganisms treated by photo-activated disinfection (PAD) in combined periodontal-endodontic (perio-endo) lesions would be exposed to sub-lethal doses of PAD (sPAD). This study evaluated the effect of sPAD using toluidine blue O (TBO) in combination with diode laser irradiation on the growth and biofilm-formation ability of Porphyromonas gingivalis as an endo-periodontal pathogen. Methods The antibacterial and antibiofilm potential of sPAD against P. gingivalis was analyzed at sub-lethal doses of TBO and irradiation time of diode laser on a colony-forming unit and crystal violet assays, respectively. Results TBO-mediated PAD, using 6.25–100 μg/mL at a fluency of 171.87 J/cm2 and 12.5–100 μg/mL at a fluency of 137.5 J/cm2, showed a significant dose-dependent reduction in P. gingivalis growth when compared to the control. TBO-mediated PAD showed a significantly inhibitory effect on biofilm formation in P. gingivalis than TBO-PAD at sub-lethal levels. Conclusion High doses of sPAD revealed antibacterial and antibiofilm potential activity, whereas lower doses of sPAD had conflicting results. Therefore, when PAD is prescribed in combined perio-endo lesions treatment, the dose of PAD used in vivo should be taken into account. © 2016 Elsevier B.V.
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