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The Effect of Antimicrobial Photodynamic Therapy Using Chlorophyllin-Phycocyanin Mixture on Enterococcus Faecalis: The Influence of Different Light Sources Publisher



Chiniforush N1, 2 ; Pourhajibagher M3 ; Parker S2 ; Benedicenti S2 ; Bahador A4 ; Salagean T5 ; Bordea IR6
Authors

Source: Applied Sciences (Switzerland) Published:2020


Abstract

The purpose of this study was to evaluate the in vitro effect of the chlorophyllin-phycocyanin mixture (Photoactive+) as a photosensitizer (PS) during antimicrobial photodynamic therapy (aPDT) on the count of Enterococcus faecalis (E. faecalis) using different light sources. The antimicrobial effect of aPDT with chlorophyllin-phycocyanin mixture using different light sources including diode laser (λ = 660 nm), diode laser (λ = 635 nm), LED (λ = 450 - 30 nm) alone or in combination was assessed using microbial cell viability assay against E. faecalis. In addition, the cell cytotoxicity of Photoactive+ was assessed on human gingival fibroblast (HuGu) cells by MTT assay; E. faecalis growth when treated by both red wavelengths (635 nm, 660 nm) and combination of LED (420-480 nm) and red wavelengths (635 nm, 660 nm), significantly reduced compared to the control group (p < 0.05). There was no significant reduction in the number of viable cells exposed to Photoactive+ compared to the control group (p < 0.05). This study shows that the application of chlorophyllin-phycocyanin mixture and irradiation with emission of red light achieved a better result for bacterial count reduction, compared to a control. This component can be applied safely due to very negligible cytotoxicity. © 2020 by the authors.
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