Evaluation of Candida Colonization Index, Molecular Identification, and Antifungal Susceptibility Pattern of Candida Species Isolated From Critically Ill Pediatric Patients: A Single-Center Study in Iran Publisher



Davari A¹ ; Hedayati MT^{2, 3} ; Jafarzadeh J⁴ ; Nikmanesh B⁵ ; Nabili M⁶ ; Hamidieh AA⁷ ; Abastabar M^{2, 3} ; Ahmadi N⁸ ; Alhatmi AMS^{9, 10} ; Moazeni M^{2, 3}
Source: Current Medical Mycology Published:2022

Abstract

Background and Purpose: Given the high mortality rate of invasive candidiasis in hospitalized pediatric patients, it is crucial to establish a predictive system to achieve early diagnosis and treatment of patients who are likely to benefit from early antifungal treatment. This study aimed to assess the Candida colonization index, species distribution, and antifungal susceptibility pattern of Candida strains isolated from pediatric patients with high Candida colonization index (CI) Materials and Methods: This study was carried out at the Children’s Medical Center in Tehran-Iran. In total, 661 samples were collected from 83 patients. The Candida CI was calculated according to the descriptions of previous studies. The isolates were identified using polymerase chain reaction-based techniques. The Clinical and Laboratory Standard Institute protocol M60 was used to conduct the antifungal susceptibility test. Results: A colonization index greater than 0.5 was confirmed in 29 cases (58% of positive samples) with two children developing candidemia. Candida albicans (n=53, 49.5%) was the most common Candida species in patients with CI > 0.5. Except for acute lymphoblastic leukemia, no risk factors were linked to a high index in colonized children (P > 0.05). Twelve isolates (7.01%) were multi-azole resistant with high MICs against both isavuconazole and ravuconazole and seven strains (4.09%) were echinocandins resistant. Conclusion: In pediatric intensive care units, patients are at risk of fungal infection, particularly candidemia. In this study, more than half of the children with positive yeast cultures had CI > 0.5, and 6.8% developed candidemia. 2022, Published by Mazandaran University of Medical Sciences on behalf of Iranian Society of Medical Mycology and Invasive Fungi Research Center.