Tehran University of Medical Sciences

Science Communicator Platform

Stay connected! Follow us on X network (Twitter):
Share By
A Bioassay-Guided Fractionation Scheme for Characterization of New Antibacterial Compounds From Prosopis Cineraria Aerial Parts



Neghabihajiagha M1 ; Aliahmadi A2 ; Taheri MR3 ; Ghassempour A3 ; Irajian G4 ; Rezadoost H3 ; Feizabadi MM1
Authors

Source: Iranian Journal of Microbiology Published:2016

Abstract

Background and Objectives:Due to the importance of finding of new antibacterial agents, the antibacterial properties of Prosopis cinerariaaerial parts were investigated using a bioassay guided fractionation scheme. Materials and Methods:The organic extract was prepared via maceration in methanol, followed by the fractionation using n-hexane and ethyl acetate. The MICs of fractions were determined against some human pathogenic bacteria using broth micro-dilution assay. The primary characterization and identification of bioactive substance(s) was based on a bio-autograph- ical method using HPTLC and flash chromatography in parallel with agar overlay assays. Finally the exact mass of effective compound(s) was determined by LC-MS. Results:The best antibacterial activities were related to the ethyl acetate fraction. The effective antibacterial compound of the plant were 2 substances with molecular weight of 348 and 184 Dalton that inhibited the growth of assessed Gram positive bacteria with MIC values lower than 125 to 62.5 µg/ml synergistically. Conclusion: Further analysis using nuclear magnetic resonance could reveal the exact structure of these two antibacterial substances. These 2 effective antibacterial compounds could be applied as lead compound for synthesis of new antibacterial agents. © 2016, Tehran University of Medical Science. All Rights reserved.
Related Docs
View other Related Docs
1. Monoterpene Isolated From the Essential Oil of Trachyspermum Ammi Is Cytotoxic to Multidrug-Resistant Pseudomonas Aeruginosa and Staphylococcus Aureus Strains, Revista da Sociedade Brasileira de Medicina Tropical (2016)
2. Antibacterial Activity of Silver Nanoparticles and Their Combination With Zataria Multiflora Essential Oil and Methanol Extract, Jundishapur Journal of Microbiology (2016)
3. Antibacterial Components of Levisticum Officinale Koch Against Multidrug-Resistant Mycobacterium Tuberculosis, Pharmaceutical Sciences (2020)
Experts (# of related papers)
View all Related Experts
Nasrin Samadi (3)
Mahnaz Khanavi (3)
Other Related Docs
4. Anti-Helicobacter Pylori Compounds From Oliveria Decumbens Vent. Through Urease Inhibitory In-Vitro and In-Silico Studies, Iranian Journal of Pharmaceutical Research (2021)
5. Analysis of Essential Oil Composition and Antimicrobial Effect of Stachys Discolor Subsp. Mazandarana, Traditional and Integrative Medicine (2020)
6. Antimicrobial Effects of Ethanol and Acetone Extracts of Plantago Major Seed on Streptococcus Mutans, Journal of Research in Dental and Maxillofacial Sciences (2023)
7. In Vitro Antibacterial Activities of Essential Oils and Extracts of Six Herbals Against Gram-Positive and Gram-Negative Bacteria, Toxicology and Environmental Health Sciences (2023)
8. Surveying the Chemical Composition and Antibacterial Activity of Essential Oils From Selected Medicinal Plants Against Human Pathogens, Iranian Journal of Microbiology (2020)
9. Study on the Chemistry Identification and Assessment of Antioxidant and Antibacterial Activity of the Biologically Active Constituents From the Roots of Arnebia Euchroma for Promising Application in Nanomedicine and Pharmaceutical, Biointerface Research in Applied Chemistry (2022)
10. Antimicrobial Activity of Endophytic Bacterial Populations Isolated From Medical Plants of Iran, Iranian Journal of Microbiology (2017)
11. Consumption of Non-Antibacterial Drugs May Have Negative Impact on Helicobacter Pylori Colonization in the Stomach, Heliyon (2024)
12. Antibacterial, Antifungal and Antioxidant Activities of Bee Glue Ethanol and Aqueous Extracts, Journal of Biological Research (Italy) (2017)
13. In Vitro Antimicrobial and Acetylcholinesterase Inhibitory Activities of Coumarins From Ferulago Carduchorum, Medicinal Chemistry Research (2016)
14. A Study of Karabaghian Skullcap (Scutellaria Platystegia Juz.): Antioxidant and Antibacterial Activity Assays, Essential Oil Analysis, and Isolation of Its Phenolic Compounds, Jundishapur Journal of Natural Pharmaceutical Products (2022)
15. Thymol and Carvacrol Against Klebsiella: Anti-Bacterial, Anti-Biofilm, and Synergistic Activities—A Systematic Review, Frontiers in Pharmacology (2024)
16. New Coumarin Derivatives From Ferula Pseudalliacea With Antibacterial Activity, Natural Product Research (2016)
17. Oliveria Decumbens, a Bioactive Essential Oil: Chemical Composition and Biological Activities, Iranian Journal of Pharmaceutical Research (2019)
18. A Single Key for Many Doors? Unlocking the Bio-Chemical Potential of Lactobacillus Bulgaricus Metabolic Postbiotic, Probiotics and Antimicrobial Proteins (2025)
19. Chemical Composition and Antibacterial Activity of the Essential Oils From Flower, Leaf and Stem of Ferula Cupularis Growing Wild in Iran, Pharmaceutical Biology (2015)
20. Investigation of Peppermint (Mentha Piperita) Extract on the Inhibition of Biofilm Formation by Acinetobacter Baumannii Strains Isolated From Clinical Samples, Iranian Journal of Pharmaceutical Research (2025)