Significance of Il-1Ra Polymorphism in Iranian Patients With Inflammatory Bowel Disease Publisher Pubmed



Daryani NE¹ ; Sadr M² ; Moossavi S³ ; Shahkarami S² ; Soltani S² ; Farhadi E⁴ ; Rezaei N^{2, 5}
Source: Digestive Diseases and Sciences Published:2015

Abstract

Background and Aim: Interleukin (IL)-1 family members play an important role in the pathogenesis of inflammatory bowel disease (IBD). There are conflicting results regarding the association of IL-1 gene cluster single nucleotide polymorphisms (SNPs) with IBD and its clinical features. The aim of this study was to examine IL-1α −889 C/T, IL-1β −511 C/T, IL-1β +3962 C/T, IL-1R Pst-I1970 C/T, and IL-1RA Mspa-I11100 C/T SNPs in Iranian patients. Methods: In this study, SNPs of IL-1 family members were investigated in 75 patients with IBD (40 CD and 35 UC), using polymerase chain reaction with sequence-specific primers method. Results: IL-1β −511 CC genotype was significantly less present in UC compared to controls, while IL-1RA Mspa-I11100 CC was significantly associated with both Crohn’s disease (CD) and ulcerative colitis (UC). IL-1α −889 TT genotype was more frequently associated with extraintestinal manifestations. A significant association was observed between IL-1β +3962 TT genotype and the disease activity in IBD. IL-1RA Mspa-I11100 CC was significantly less frequent in CD patients who need immunosuppressive therapy. IL-1RA Mspa-I11100 CT was associated with earlier age of onset in IBD, while TT genotype was associated with higher age of onset in IBD. Conclusions: IL-1 SNPs seem to be associated with IBD and could affect the disease severity as well. © 2014, Springer Science+Business Media New York.