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Apoptotic Effect of Resveratrol on Human T-All Cell Line Ccrf-Cem Is Unlikely Exerted Through Alteration of Bax and Bcl2 Promoter Methylation Publisher Pubmed



Zadi Heydarabad M1 ; Vatanmakanian M2 ; Abdolalizadeh J3 ; Mohammadi H1 ; Azimi A4 ; Mousavi Ardehaie R5 ; Movasaghpour A6 ; Farshdousti Hagh M3
Authors

Source: Journal of Cellular Biochemistry Published:2018


Abstract

One of the fundamental barriers leading to failure of leukemia therapy is the resistance against conventional chemotherapies, common modality used to cure leukemia. Having the potential to trigger apoptosis in various human leukemia cell lines, resveratrol is regarded as a robust agent in chemotherapy regimens. The current study was aimed to assess whether the apoptotic effect of resveratrol on T-cell acute lymphoblastic leukemia cell line, CCRF-CEM, is exerted through DNA methylation of BAX and BCL2 gene promoters. For this purpose, the CCRF-CEM cells were treated by resveratrol under standard cell culture. To analyze the promoter DNA methylation changes, we used methylation-specific polymerase chain reaction technique following the resveratrol treatment at different dosages and time intervals. Based on our previous study, the resveratrol treatment can trigger apoptosis in CCRF-CEM cell line via upregulation of apoptotic BAX gene and downregulation of antiapoptotic BCL2 gene. Despite these alterations in gene expression, the current study reveals no changes in DNA methylation patterns of subjected genes following the resveratrol treatment. Unchanged status of DNA methylation of BAX and BCL2 genes may suggest that resveratrol causes the gene expression changes through a distinct mechanism which requires further studies to be understood. © 2018 Wiley Periodicals, Inc.
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