Single-Walled Carbon Nanotube, Multi-Walled Carbon Nanotube and Fe2o3 Nanoparticles Induced Mitochondria Mediated Apoptosis in Melanoma Cells Publisher Pubmed



Naserzadeh P¹ ; Ansari Esfeh F¹ ; Kaviani M¹ ; Ashtari K⁴ ; Kheirbakhsh R² ; Salimi A³ ; Pourahmad J¹
Source: Cutaneous and Ocular Toxicology Published:2018

Abstract

Purpose: Nanomaterials (NM) exhibit novel anticancer properties. Materials and methods: The toxicity of three nanoparticles that are currently being produced in high tonnage including single-walled carbon nanotube (SWCNT), multi-walled carbon nanotube (MWCNT) and Fe2O3 nanoparticles, were compared with normal and melanoma cells. Results: All tested nanoparticles induced selective toxicity and caspase 3 activation through mitochondria pathway in melanoma cells and mitochondria cause the generating of reactive oxygen species (ROS), mitochondrial membrane potential decline (MMP collapse), mitochondria swelling, and cytochrome c release. The pretreatment of butylated hydroxytoluene (BHT), a cell-permeable antioxidant and cyclosporine A (Cs. A), a mitochondrial permeability transition (MPT), pore sealing agent decreased cytotoxicity, caspase 3 activation, ROS generation, and mitochondrial damages induced by SWCNT, MWCNT, and IONPs. Conclusions: Our promising results provide a potential approach for the future therapeutic use of SWCNT, MWCNT, and IONPs in melanoma through mitochondrial targeting. © 2017 Informa UK Limited, trading as Taylor & Francis Group.