Tehran University of Medical Sciences

Science Communicator Platform

Stay connected! Follow us on X network (Twitter):
Share By
Rare Codons Effect on Expression of Recombinant Gene Cassette in Escherichia Coli Bl21(De3) Publisher



Esmaeilibandboni A1 ; Honari H2 ; Safaei S3
Authors

Source: Asian Pacific Journal of Tropical Disease Published:2017


Abstract

Objective: To demonstrate the sensitivity of expression of fusion genes to existence of a large number of rare codons in recombinant gene sequenced. Methods: Primers for amplification of cholera toxin B, Shiga toxin B and gfp genes were designed by Primer3 software and synthesized. All of these 3 genes were cloned. Then the genes were fused together by restriction sites and enzymatic method. Two linkers were used as a flexible bridge in connection of these genes. Results: Cloning and fusion of cholera toxin B, Shiga toxin B and gfp genes were done correctly. After that, expression of the recombinant gene construction was surveyed. Conclusions: According to what was seen, because of the accumulation of 12 rare codons of Shiga toxin B and 19 rare codons of cholera toxin B in this gene cassette, the expression of the recombinant gene cassette, in Escherichia coli BL21, failed. © 2017 by the Asian Pacific Journal of Tropical Disease.
Related Docs
View other Related Docs
1. Colonization and Investigation of Vibrio Cholera Recombination Protein in E-Coli, International Journal of Engineering and Technology(UAE) (2018)
2. Genetic Diversity and Antibiotic Resistance Patterns of Escherichia Coli Isolates Causing Septicemia: A Phylogenetic Typing and Pfge Analysis, Diagnostic Microbiology and Infectious Disease (2025)
3. High-Throughput Genomic and Proteomic Interpretation of Gene Duplication in Vibrio Cholera Genomes: An in Silico Study, Informatics in Medicine Unlocked (2023)
Experts (# of related papers)
View all Related Experts
Abbas Bahador (3)
Masoumeh Douraghi (1)
Other Related Docs
4. Designing, Optimizing, and Structure Prediction of Chimeric Protein Ctb-Ipad for Expression in E.Coli, Biosciences Biotechnology Research Asia (2015)
5. The Effect of Bifidobacterium Longum on Expression of Hfq in Escherichia Coli O157:H7, Iranian Journal of Medical Microbiology (2024)
6. Cloning, Expression and Purification of Hpaa Recombinant Protein of Helicobacter Pylori As a Vaccine Candidate, Journal of Pure and Applied Microbiology (2016)
7. Cloning, Expression, and Purification of Hpaa-Caga Fusion Recombinant Protein of Helicobacter Pylori in E. Coli Bl 21 Strain, Gene Reports (2019)
8. Construction, Cloning, and Expression of Caga Recombinant Protein of Helicobacter Pylori, Avicenna Journal of Medical Biotechnology (2020)
9. The Impact of Primer Sets on Detection of the Gene Encoding Biofilm-Associated Protein (Bap) in Acinetobacter Baumannii: In Silico and In Vitro Analysis, Letters in Applied Microbiology (2017)
10. Targeting of Cholera Toxin a (Ctxa) Gene by Zinc Finger Nuclease: Pitfalls of Using Gene Editing Tools in Prokaryotes, Research in Pharmaceutical Sciences (2020)
11. Characterization of Toxin-Antitoxin (Ta) Systems in Pseudomonas Aeruginosa Clinical Isolates in Iran, Jundishapur Journal of Microbiology (2016)
12. Expression of Salmonella Typhimurium and Escherichia Coli Flagellin Protein and Its Functional Characterization As an Adjuvant, Microbial Pathogenesis (2018)
13. Virulence Profiling and Genetic Relatedness of Shiga Toxin-Producing Escherichia Coli Isolated From Humans and Ruminants, Comparative Immunology# Microbiology and Infectious Diseases (2015)