Association Between the Presence of Crispr-Cas System Genes and Antibiotic Resistance in Klebsiella Pneumoniae Isolated From Patients Admitted in Ahvaz Teaching Hospitals Publisher Pubmed



Montazeri EA^{1, 2} ; Saki M^{1, 2} ; Savari M^{1, 2} ; Meghdadi H² ; Akrami S^{1, 3}
Source: BMC Infectious Diseases Published:2024

Abstract

Background: This study aims to investigate the frequency of cas1 and cas3 and CRISPR1,2,3 genes in Klebsiella pneumoniae isolates, as well as their connection with antibiotic resistance. Materials and methods: 106 K. pneumoniae isolates were identified by biochemical assays and PCR. The susceptibility to antibiotics was determined by Kirby–Bauer disk diffusion method. Screening of ESBLs was undertaken by using double disk diffusion and standard disk diffusion methods. The E-test and mCIM techniques was used to confirm the disc diffusion-based carbapenem resistance profiles. CRISPR-Cas system genes were identified using PCR. Results: ESBL production was found in 19% of isolates. Carbapenemase production was found in 46% of the isolates. Furthermore, the bacteria were classified as multidrug (76%), extensively drug-resistant (4%), or pan-drug-resistant (2%). When CRISPR/Cas systems were present, antibiotic resistance was lower; conversely, when they were absent, resistance was higher. Conclusions: If the CRISPR/Cas modules aren’t present, the bacteria can still acquire foreign DNA, including antibiotic resistance genes. K. pneumoniae isolates with a CRISPR-Cas system were less likely to carry antibiotic-resistance genes than those lacking this defense system. © The Author(s) 2024.