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Development of a New Immunochromatographic Assay Using Gold Nanoparticles for Screening of Iga Deficiency Pubmed



Goudarzi S1 ; Ahmadi A2, 4 ; Farhadi M1 ; Kamrava SK1 ; Saghafi S3 ; Omidfar K2, 4
Authors

Source: Iranian Journal of Allergy# Asthma and Immunology Published:2015


Abstract

A new competitive immunochromatography (ICG) strip test based on polyclonal antibody (pAb) conjugated with gold nanoparticles (NPs) was developed and its applications for primary screening of immunoglobulin (Ig) A in serum were evaluated. Nanocolloidal gold as the detection reagent, with an average particle diameter of 20 nm, was synthesized and labelled pAb. The antibody-nanocolloidal gold probe was applied on the conjugate pad, and human IgA was immobilized on a nitrocellulose membrane as the capture reagent to prepare the ICG strip test. It took only 10 minutes to accomplish a semi-quantitative detection of serum IgA in this assay. In the optimized investigational conditions, the ICG strip test could distinguish human serum IgA in the range from 1 to 270 ng/mL with a detection limit of 5 ng/mL. The reliability of testing procedures was examined by performing the ICG strip test with 11 serum samples and comparing the results with those obtained via enzyme-linked immunosorbent assay (ELISA). The ICG strip was sufficiently sensitive and accurate for a rapid screening of IgA in human serum. Copyright © Winter 2015.
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