Reduced Expression of Il10, Stat3, Hoxa10, and Itgb3 in the Embryo Implantation Site of Rat Model With Prenatal Androgen-Induced Polycystic Ovary Syndrome Publisher Pubmed



Changaei M¹ ; Javidan M¹ ; Ramezani Tehrani F² ; Mosaffa N³ ; Noroozzadeh M² ; Hosseinzadeh R¹ ; Rajaei S¹
Source: American Journal of Reproductive Immunology Published:2023

Abstract

Aims: Impaired implantation due to the reduced endometrial receptivity considers an etiology for infertility in polycystic ovary syndrome (PCOS). In this context, we aimed to compare the expression of interleukin 10 (Il10), homeobox A10 (Hoxa10), signal transducer and activator of transcription 3 (Stat3), and β3-integrin (Itgb3) in the embryo implantation site of a prenatally-androgenized rat model of PCOS before and during gestation. Materials and Methods: PCOS rat model was created by the injection of testosterone prenatally. The uterine tissues were collected before pregnancy (day 0) and on days 0.5, 4.5, 5.5, and 8.5 of gestation in the PCOS rat model and controls (n = 6; each group). RNA was extracted from the uterine samples and reverse transcribed to cDNA. Expression levels of Il10, Stat3, Hoxa10, and Itgb3 were measured using SYBR Green real-time RT-PCR and compared between the two groups. Findings: PCOS rats showed decreased expression levels of the Il10 on day 8.5 compared to control rats. The mRNA levels of Hoxa10, Itgb3, and Stat3 were significantly decreased in the PCOS group on day 0 as well as on days 0.5, 4.5, 5.5, and 8.5 for Hoxa10, Itgb3, and Stat3. Significance: The decreased gene expression of Il10, Hoxa10, Stat3, and Itgb3 in the PCOS rat model indicates the importance of the Il10 signaling axis as one of the possible disrupted mechanisms of endometrial receptivity in PCOS. © 2023 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.