Potential Efficacy of Lactobacillus Casei Ibrc_M10711 on Expression and Activity of Insulin Degrading Enzyme But Not Insulin Degradation Publisher Pubmed



Neyazi N^{1, 2} ; Mohammadi Farsani T¹ ; Nouri Z¹ ; Ghahremani MH^{3, 4} ; Khorramizadeh MR^{1, 5} ; Tajerian R⁶ ; Motevaseli E^{4, 7}
Source: In Vitro Cellular and Developmental Biology - Animal Published:2017

Abstract

Type 2 diabetes (T2D) is a condition with insufficient insulin production or in the setting of insulin resistance with many origins including intestinal microbiota-related molecular mechanism. Insulin-degrading enzyme (IDE) is responsible for insulin breakdown in various tissues and is known as a potential drug target for T2D. Here, we assessed the effects of cell-free supernatant (CFS) and UV-killed Lactobacillus casei IBRC_M10711 on IDE expression, IDE activity, and insulin degradation in Caco-2 cell line. It was found that CFS and UV-killed L. casei IBRC_M10711 led to lower expression of IDE. UV-killed L. casei IBRC_M10711 significantly inhibited IDE activity but CFS did not. Insulin degradation was affected with none of them. In conclusion, L. casei IBRC_M10711 is effective on IDE expression and its activity, but not on insulin degradation. Future studies are recommended to explore the effect of this probiotic on other substrates of IDE. © 2016, The Society for In Vitro Biology.