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The Effect of Mirna Mimic Hsa-Mir-7704 on In-Vitro Replication of Herpes Simplex Virus Type 1 Publisher

Summary: Scientists report a microRNA reduces herpes virus replication in cells, offering a new way to fight infections. #HerpesResearch #AntiviralTherapy

Shabani M1 ; Nasresfahani B1 ; Sadeghehdaei B2 ; Moghim S1 ; Mirzaei A1 ; Sharifi MR3
Authors

Source: Journal of Isfahan Medical School Published:2018


Abstract

Background: Herpes simplex virus type 1 (HSV-1) infections are of the most common diseases in human population. HSV-1 can cause subclinical to severe diseases, especially in immunocompromised patients. There are few anti-herpes drugs for treatment of HSV-1 infection. Acyclovir is one of the most important drugs. The extensive use of this drug has led to the development of resistant strains. Therefore, development of new anti-herpes drugs with different mechanisms is noticeable. This study aimed to use microRNAs as a novel method for inhibiting HSV-1 infection. Methods: Synthesized miRNA mimics hsa-miR-7704 (miR-SX1) were transfected into Hela cells, and then infected with HSV-1. Cellular morphological changes were observed 24 hours post-infection by inverted microscope, and photographed. Viral titers were measured using 50% tissue culture infective dose (TCID 50 ) method. Findings: miR-SX-1-transfected cells produced low-titer HSV-1, without affecting cell viability. Conclusion: The data suggest that miR-SX1 inhibits HSV-1 replication, and may provide an alternative mechanism to prevent HSV-1 infection. © 2018, Isfahan University of Medical Sciences(IUMS). All rights reserved.
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Sharareh Moghim (4)
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