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Platelet Rich Fibrin and Simvastatin-Loaded Pectin-Based 3D Printed-Electrospun Bilayer Scaffold for Skin Tissue Regeneration Publisher Pubmed



Tavakoli M1 ; Almusawi MH2 ; Kalali A3 ; Shekarchizadeh A4 ; Kaviani Y5 ; Mansouri A6 ; Nasiriharchegani S7 ; Kharazi AZ8 ; Sharifianjazi F9 ; Sattar M10 ; Varshosaz J11 ; Mehrjoo M12 ; Najafinezhad A7 ; Mirhaj M1
Authors

Source: International Journal of Biological Macromolecules Published:2024


Abstract

Designing multifunctional wound dressings is a prerequisite to prevent infection and stimulate healing. In this study, a bilayer scaffold (BS) with a top layer (TL) comprising 3D printed pectin/polyacrylic acid/platelet rich fibrin hydrogel (Pec/PAA/PRF) and a bottom nanofibrous layer (NL) containing Pec/PAA/simvastatin (SIM) was produced. The biodegradable and biocompatible polymers Pec and PAA were cross-linked to form hydrogels via Ca2+ activation through galacturonate linkage and chelation, respectively. PRF as an autologous growth factor (GF) source and SIM together augmented angiogenesis and neovascularization. Because of 3D printing, the BS possessed a uniform distribution of PRF in TL and an average fiber diameter of 96.71 ± 18.14 nm was obtained in NL. The Young's modulus of BS was recorded as 6.02 ± 0.31 MPa and its elongation at break was measured as 30.16 ± 2.70 %. The wound dressing gradually released growth factors over 7 days of investigation. Furthermore, the BS significantly outperformed other groups in increasing cell viability and in vivo wound closure rate (95.80 ± 3.47 % after 14 days). Wounds covered with BS healed faster with more collagen deposition and re-epithelialization. The results demonstrate that the BS can be a potential remedy for skin tissue regeneration. © 2024 Elsevier B.V.
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