Microrna-200C Overexpression in Cancer-Associated Fibroblasts Decreases Interleukin-2 Secretion Publisher

Summary: Can a tiny RNA dial down cancer's inflammatory sidekicks? A study found miR-200c-3p transfection cuts IL-2 secretion in CAFs and NFs—potentially curbing tumor promotion by taming fibroblast signals. #CancerMicroenvironment #miRNA

Shariati L^{1, 2} ; Vaseghi G³ ; Vaziri N⁴ ; Shenavar N⁵ ; Zarrabi A⁶ ; Haghjooy Javanmard S⁵
Source: Clinical and Translational Discovery Published:2022

Abstract

miR-200c-3p is demonstrated to play the role of tumour suppressor in different tumours. However, the miR-200c-3p biological function in normal fibroblast (NF) and cancer-associated fibroblast (CAF) remains unclear. This investigation aims to study the regulatory role of miR-200c-3p in the secretion of Interleukin-2 (IL-2) in CAF and NF. CAFs and NFs were isolated from tumour and normal tissue specimens respectively. Immunocytochemistry was used to confirm the presence of a fibroblast specific marker, alpha-actin smooth muscle, in NFs and CAFs. NF and CAF were transfected with scramble and miR-200c-3p utilizing the lipofectamine 2000 reagent. The protein levels of IL-2 were measured in CAFs, NFs, and transfected groups with miR-200c-3p and scrambled using an IL-2 enzyme-linked immunoassay kit. miR-200c decreased secretion of IL-2 in transfected CAF and NF compared to controls. Results elucidated that transfection of MiR-200c-3p can decrease the IL-2 secretion and consequently reduce IL-induced tumourigenic manner in the CAF. © 2022 The Authors. Clinical and Translational Discovery published by John Wiley & Sons Australia, Ltd on behalf of Shanghai Institute of Clinical Bioinformatics.